摘要
目的利用孟德尔随机化(MR)分析探究弓形虫感染与脑炎的因果关系,并通过免疫组化观察脑组织中的弓形虫包囊分布和炎症情况。方法获取弓形虫感染和脑炎的全基因组关联分析数据,筛选出单核苷酸多态性(SNPs)位点,以逆方差加权法为主进行MR分析,以OR值及95%CI评价弓形虫感染与脑炎之间的因果关系。利用异质性检验、水平多效性检验、留一法进行质量控制。利用Wh6弓形虫包囊感染的小鼠脑组织切片进行免疫组化染色,使用Image J软件进行图片分析。结果筛选出29个弓形虫感染与脑炎存在相关性的SNP,IVW方法结果提示弓形虫感染使得脑炎的患病分险为原来的0.98倍(OR=0.98,95%CI为0.76~1.27),显示两者无因果关系。质量控制结果提示筛选出的SNPs具有稳定可靠性。弓形虫包囊在脑组织各部位分布不同。结论弓形虫感染与脑炎具有相关性,但未有充分证据证明二者之间存在因果关系。
Objective Mendelian randomization analysis was used to explore the causal relationship of T.gondii infection and the cyst distribution and inflammation in brain tissue by immunohistochemistry.Methods Genome-wide association analysis data of T.gondii infection and encephalitis were obtained,single nucleotide polymorphisms(SNPs)were selected,Mendelian randomization analysis was conducted by inverse variance weighting,and the causal relationship between T.gondii infection and encephalitis was evaluated by OR value and 95%CI.Quality control was carried out by using heterogeneity test,horizontal multi-efficiency test and leave-one-out sensitivity test.Immunohistochemical staining was performed using brain sections of mice infected with tissue cysts of Wh6 strain for image analysis using Image J software.Results A total of 29 SNPs were associated with toxoplasmic encephalitis.The results of IVW method suggested that T.gondii infection made encephalitis risk 0.98 times higher(OR=0.98,95%CI=0.76 to 1.27),indicating no causal relationship between the two.The quality control results suggested that the selected SNPs were stable and reliable.Toxoplasma cysts were distributed in various parts of the brain tissue.Conclusion T.gondii infection and encephalitis are related,but there is no sufficient evidence to prove the causal relationship between the two.
作者
李伊凡
盛书颜
吴梦云
计永胜
姚湧
Li Yifan;Sheng Shuyan;Wu Mengyun;Ji Yongsheng;Yao Yong(Dept of Parasitology and Microbiology,School of Basic Medicine,Anhui Medical University,Hefei 230032;The First Clinical Medical College,Anhui Medical University,Hefei 230032;Division of Life Sciences and Medicine,University of Science and Technology of China,Hefei 230027)
出处
《安徽医科大学学报》
CAS
北大核心
2024年第1期28-33,共6页
Acta Universitatis Medicinalis Anhui
基金
国家自然科学基金(编号:81802026)
安徽高校自然科学研究项目(编号:KJ2019A0264、2023AH040077)。
关键词
孟德尔随机化
弓形虫
脑炎
Mendelian randomization
Toxoplasma gondii
encephalitis
