摘要
目的:探讨hsa-miR-155-3p和hsa-miR-155-5p作为系统性硬化症(systemic sclerosis,SSc)生物标志物及调控生物学行为的研究。方法:选取10例SSc患者和10例健康对照者作为研究对象,采用RT-qPCR法检测SSc患者及健康对照者外周血单个核细胞(PBMCs)中hsa-miR-155-3p和hsa-miR-155-5p表达水平。应用受试者工作特征(ROC)曲线探究其诊断价值。Pearson或Spearman分析miRNA和SSc患者临床指标的相关性。利用miRDB、Targetscan和miRDIP数据库预测hsa-miR-155-3p和hsa-miR-155-5p靶基因,并进行GO功能注释和KEGG Pathway富集分析、PPI相互作用网络构建、中心基因的筛选。结果:在SSc患者PBMCs中hsa-miR-155-3p和hsa-miR-155-5p的表达水平均明显高于健康对照者(P<0.001)。ROC曲线显示,hsa-miR-155-3p和hsa-miR-155-5p诊断SSc的曲线下面积为(AUC=1, P<0.001)。相关性分析显示,hsa-miR-155-3p、 hsa-miR-155-5p和高分辨率CT(HRCT)、中性粒细胞百分比(NEUT%)、淋巴细胞百分比(LYM%)、白球比(ALB/GLB)等临床指标相关(P<0.05)。hsa-miR-155-3p和hsa-miR-155-5p靶基因富集的信号通路与SSc纤维化、免疫、炎症的发生发展密切相关。结论:hsa-miR-155-3p及hsa-miR-155-5p可能参与了调节SSc纤维化、免疫、炎症的发生、发展,hsa-miR-155-3p及hsa-miR-155-5p有可能作为SSc临床诊断和治疗的潜在生物标志物。
Objective:This study was to investigate the role of hsa-miR-155-3p and hsa-miR-155-5p as biomarkers and regulators of biological behavior in systemic sclerosis(SSc).Methods:A total of 10 SSc patients and 10 healthy controls were selected for the study.The expression levels of hsa-miR-155-3p and hsa-miR-155-5p in peripheral blood mononuclear cells of SSc patients and healthy controls were measured using RT-qPCR.The diagnostic value of these miRNAs was explored using Receiver Operating Characteristic curve analysis.Pearson or Spearman correlation analysis was performed to assess the correlation between miR-NAs and clinical indicators in SSc patients.Potential target genes of hsa-miR-155-3p and hsa-miR-155-5p were predicted using miRDB,Targetscan,and miRDIP databases.GO functional annotation,KEGG pathway enrichment analysis,protein-protein interaction network construction,and selection of central genes were conducted.Results:The expression levels of hsa-miR-155-3p and hsa-miR-155-5p were significantly higher in PBMCs of SSc patients compared to healthy controls(P<0.001).The ROC curve analysis showed that hsa-miR-155-3p and hsa-miR-155-5p had a high diagnostic value for SSc(AUC=1,P<0.001).Correlation analysis revealed that hsa-miR-155-3p,hsa-miR-155-5p,and clinical indicators such as high-resolution CT,neutrophil percentage,lymphocyte percentage,and albumin to globulin ratio were correlated(P<0.05).The signaling pathways enriched with target genes of hsa-miR-155-3p and hsa-miR-155-5p were closely associated with the occurrence and development of SSc fibrosis,immunity,and inflammation.Conclusions:Hsa-miR-155-3p and hsa-miR-155-5p may be involved in regulating the occurrence and development of SSc fibrosis,immunity,and inflammation.They have the potential to serve as biomarkers for clinical diagnosis and treatment of SSc.
作者
王宝玥
孙晓林
王永福
WANG Baoyue;SUN Xiaolin;WANG Yongfu(Baotou Medical College,Inner Mongolia University of Science and Technology,Baotou 014010,China;Department of Rheumatology,the First Affiliated Hospital of Baotou Medical College,Inner Mongolia University of Science and Technology,Baotou 014010,China)
出处
《海南医学院学报》
CAS
北大核心
2024年第3期192-202,共11页
Journal of Hainan Medical University
基金
国家自然科学基金资助项目(81860294,81860295)
内蒙古自治区自然科学基金资助项目(2019MS08055,2021MS08045)
内蒙古自治区科技计划项目(201802089,2019GG052)。
