摘要
目的:探究化学杀菌剂单剂及其混配对高梁附球菌(E.sorghinum)的抑制活性,旨在找出抑菌效果最佳的混配组合。方法:采用Horsfall的方法设计2种化学杀菌剂的混配试验,采用菌丝生长速率法求出各配比的抑菌率和毒性比率。结果:咯菌腈对高粱附球菌的抑制活性最高,EC_(50)达0.32 mg/L;其次为咪鲜胺和氟硅唑,EC_(50)分别为0.38、0.52 mg/L。百菌清、溴菌腈和代森锰锌对病菌的抑制活性较弱,EC_(50)分别为69.24、79.51、84.40 mg/L。氟硅唑和克菌丹以6∶4的比例混配抑制效果最佳,毒性比率达1.40,以2∶8比例的混配毒性比率达1.35;咪鲜胺与咯菌腈以3∶7的比例混配,毒性比率达1.34;以5∶5的比例混配毒性比率为1.31。溴菌腈与百菌清以8∶2的比例混配毒性比率最高,为1.33,其次为7∶3的比例混配毒性比率为1.30。氟硅唑与百菌清以4∶6的比例混配,毒性比率为1.31;溴菌腈与代森锰锌以7∶3的比例混配,毒性比率为1.28。结论:不同化学杀菌剂的混配对于高粱附球菌能够表现出较好的增效抑制活性,且大多为选择性杀菌剂和保护性杀菌剂混配,能够较好发挥预防和治疗的效果,其中以氟硅唑和克菌丹的混配组合增效最佳,其次是咪鲜胺和咯菌腈的混配组合。
Objective:The inhibitory activity of single chemical fungicides and their mixed combinations against E.sorghinum was investigated in order to find out the best mixed combination of antifungal effects.Methods:The mixed test of two chemical fungicides was designed by Horsfall method.The inhibition rate of each ratio was determined by mycelial growth rate method,and the toxicity ratio was calculated.Results:Fludioxonil had the highest inhibitory activity against E.sorghinum with EC_(50) of 0.32 mg/L,followed by prochloraz and flusilazole with EC_(50) of 0.38,0.52 mg/L,respectively.The inhibitory activities of chlorothalonil,bromothalonil and mancozeb were weak,and the EC_(50) was 69.24,79.51,84.40 mg/L,respectively.The mixture of flusilazole and captan at a ratio of 6∶4 had the best inhibitory effect,with a toxicity ratio of 1.40,and the mixture at a ratio of 2∶8 had a toxicity ratio of 1.35.Prochloraz and fludioxonil were mixed at a ratio of 3∶7,and the toxicity ratio was 1.34.The toxicity ratio of 5∶5 was 1.31.The highest toxicity ratio of bromothalonil and chlorothalonil in the ratio of 8∶2 was 1.33,followed by 7∶3 toxicity ratio of 1.30.Flusilazole and chlorothalonil were mixed at a ratio of 4∶6,and the toxicity ratio was 1.31.The toxicity ratio of bromothalonil to mancozeb at 7∶3 was 1.28.Conclusion:The mixture of different chemical fungicides could show good synergistic inhibitory activity against E.sorghinum,and most of them were mixed with selective fungicides and protective fungicides,which could better play the role of prevention and treatment.Among them,the mixed combination of flusilazole and captan had the best synergistic effect,followed by the combination of prochloraz and fludioxonil.
作者
杨胜雨
段海明
孟祥涛
陈甦
周慧
YANG Shengyu;DUAN Haiming;MENG Xiangtao;CHEN Su;ZHOU Hui(College of Resource and Environment,Anhui Science and Technology University,Fengyang 233100,China;College of Agriculture,Anhui Science and Technology University,Fengyang 233100,China)
出处
《安徽科技学院学报》
2024年第1期52-57,共6页
Journal of Anhui Science and Technology University
基金
安徽省自然科学基金(2308085MC90)
作物抗逆育种与减灾国家地方联合工程实验室开放基金(NELCOF20190102)
安徽省高校自然科学研究项目(KJ2017A510,KJ2019A0814)
安徽省重点研究与开发计划项目(202104a06020001)
凤阳县科技计划项目(NY2022-05)
国家级大学生创新创业训练计划项目(202110879085)。
关键词
高粱附球菌
化学杀菌剂
混配
抑制效果
毒力测定
Epicoccum sorghinum
Fungicide
Mixture
Inhibition effect
Toxicity determination
