摘要
随着全球化学品数量的不断增加,传统的哺乳动物毒性评价方式已经无法满足化学品环境监管的需求.21世纪毒性测试转变成以高通量体外测试和非哺乳类动物模型替代方法为主的新模式.斑马鱼既有生物复杂度又具一定的分析通量,是高通量的药物筛选和化学品毒性测试中重要的动物模型.本文综述了斑马鱼在化学品高通量毒性评价中应用的重要研究进展,分析了多种自动化技术在斑马鱼高通量分析中的优势和潜力,最后探讨了斑马鱼高通量毒性测试未来的发展方向.本文为研究人员高效利用斑马鱼胚胎开展化学品安全性评价提供参考.
As the global number of chemicals continues to increase,the traditional toxicity assessment methods that employed mammalian model are no longer adequate to meet the demands of environmental regulation.Consequently,the toxicity testing methodologies employ in the 21st century have shifted to a new paradigm which is mainly based on high-throughput in vitro testing and the alternative methods in the non-mammalian models.Zebrafish,due to its biological complexity and analytical throughput,is currently an important animal model in high-throughput drug screening and toxicity testing of chemicals.In this article,we reviewed the most important research advances of high-throughput toxicity assessment of chemicals by the zebrafish model.We also summarized the advantages and potential of multiple automated techniques in the workflow of high-throughput analysis by zebrafish.Finally,we proposed several perspectives for future directions of high-throughput toxicity testing by zebrafish.This review will provide systematic supports to researchers to efficiently use zebrafish embryo in safety assessment of chemicals.
作者
王俊
刘力
徐颖君
刘俊杰
程波
张蒙
庞少臣
梁勇
宋茂勇
WANG Jun;LIU Li;XU Yingjun;LIU Junjie;CHENG Bo;ZHANG Meng;PANG Shaochen;LIANG Yong;SONG Maoyong(School of Environmental Ecology and Biological Engineering,Wuhan Institute of Technology,Wuhan,430205,China;Hubei Key Laboratory of Environmental and Health Effects of Persistent Toxic Substances,School of Environment and Health,Jianghan University,Wuhan,430056,China;Key Laboratory of Environmental Nanotechnology and Health Effects,Research Center for Eco-Environmental Sciences,Chinese Academy of Sciences,Beijing,100085,China)
出处
《环境化学》
CAS
CSCD
北大核心
2024年第1期47-59,共13页
Environmental Chemistry
基金
国家自然科学基金(22276074)
湖北省自然科学基金(2022CFB487)资助。
关键词
高通量
毒性筛查
风险评估
斑马鱼
化学品
high-throughput
toxicity screening
risk evaluation
zebrafish
chemicals
