摘要
为研究蛋白质ADP核糖基化对宰后成熟初期秦川牛肉线粒体功能及肉品质的影响,以20µmol/L Rucaparib(ADP核糖聚合酶1(poly(ADP-ribose)polymerase 1,PARP1)抑制剂)处理的秦川牛背最长肌为研究对象,测定贮藏0 h、6 h、12 h、2 d、4 d、8 d对照组和抑制剂处理组样品的线粒体相关指标及肌原纤维小片化指数(myofibril fragmentation index,MFI)、剪切力、pH值等品质指标,并采用免疫印迹(Western Blot)法检测PARP1、肌间线蛋白表达水平。结果表明:0 h~8 d(12 h除外)抑制剂组活性氧含量显著低于对照组(P<0.05),宰后0~12 h抑制剂组Caspase-3活性、MFI显著低于对照组(P<0.05);在2~4 d抑制剂组线粒体膜电位较对照组高,4~8 d抑制剂处理组琥珀酸脱氢酶(succinate dehydrogenase,SDH)活性显著高于对照组(P<0.05)。说明抑制表征ADP核糖基化反应进行的PARP1后,线粒体膜电位下降变缓,SDH活性升高,这一定程度上维持了线粒体功能,使MFI下降、肌间线蛋白降解变缓,从而延缓肉品嫩化。
This study addressed the effect of adenosine 5’-diphosphate(ADP)ribosylation on the mitochondrial function and quality of Qinchuan cattle meat during early postmortem aging.The Longissimus dorsi muscle of Qinchuan cattle treated with 20µmol/L rucaparib,a poly(ADP-ribose)polymerase 1(PARP1)inhibitor,were evaluated for mitochondrial indices,myofibrillar fragmentation index(MFI),shear force,pH,and other quality indices after being stored for 0 h,6 h,12 h,2 d,4 d,and 8 d and the expression levels of PARP1 and desmin were detected using Western blot.The results showed that the content of reactive oxygen species(ROS)in the treated group was significantly lower than that in the control group(P<0.05)during 0 h–8 d(except 12 h)after slaughter.The caspase-3 activity and MFI in the treated group were significantly lower than those in the control group(P<0.05)during 0–12 h.Mitochondrial membrane potential during 2–4 d as well as succinate dehydrogenase(SDH)activity during 4–8 d were significantly higher in the treated group than in the control group(P<0.05).These findings indicate that PARP1 inhibition,which characterizes ADP ribosylation,can slow down the decrease in mitochondrial membrane potential and increase SDH activity,preserving mitochondrial function to some extents and delaying the decrease in MFI and the degradation of desmin and thereby meat tenderization.
作者
伏棋画
李亚蕾
罗瑞明
王雪蓉
马旭华
FU Qihua;LI Yaei;LUO Ruiming;WANG Xuerong;MA Xuhua(School of Food and Wine,Ningxia University,Yinchuan 750021,China)
出处
《食品科学》
EI
CAS
CSCD
北大核心
2024年第1期8-14,共7页
Food Science
基金
国家自然科学基金地区科学基金项目(32160535)
宁夏回族自治区重点研发项目(2017BY068)。
关键词
秦川牛
背最长肌
ADP核糖基化
细胞凋亡
Qinchuan cattle
Longissimus dorsi muscle
adenosine 5’-diphosphate ribonylation
apoptosis
