摘要
铁蛋白(ferritin)是由24个亚基自组装而成的中空笼形结构蛋白,具有良好的水溶性、稳定性、生物相容性和可逆自组装特性。在生物体内,铁蛋白在铁的吸收、转运、氧化和贮存中扮演重要角色,具有调节体内铁代谢平衡的功能。近年来,由于铁蛋白具有纳米尺度的笼形结构,而且容易通过化学和生物等方法进行结构改造和修饰,使得其被广泛用于检测传感元件的构建。该文对铁蛋白分子的结构与功能进行简要介绍,总结了铁蛋白在构建检测传感元件中的2种基本策略,并重点回顾了基于铁蛋白传感元件在食品真菌毒素、重金属离子、病毒、过氧化氢等有毒有害物质检测中的研究进展,以期为铁蛋白在食品检测领域的应用提供研究思路。
Ferritin is composed of 24 subunits that are self-assembled into a cage-like protein nanocage and possess good water solubility,stability,biocompatibility,and reversible self-assembly features.In biology,ferritin proteins play an important role in iron absorption,transport,oxidation,and storage,and participate in iron metabolism.In recent years,due to their nanoscale particle size and robust structure that is amenable to chemical and genetic engineering,ferritin nanocages have been widely used for the construction of biosensors in food,medical,and material sciences.In this paper,by starting with a brief introduction to the structure and function of ferritin nanocages,two basic strategies for the construction of ferritin-based sensors were summarized.Subsequently,the research progress on the ferritin nanocage-based sensors for the detection of food mycotoxins and harmful substances including food toxins,heavy metal ions,viruses,and hydrogen peroxide were reviewed.The purpose of this article was to provide insight into the construction of a ferritin-based sensor for the detection of food hazardous substances.
作者
韩雪儿
谢江
虎梦吉
马良
郭婷
张宇昊
尚永彪
陈海
HAN Xueer;XIE Jiang;HU Mengji;MA Liang;GUO Ting;ZHANG Yuhao;SHANG Yongbiao;CHEN Hai(College of Food Science,Southwest University,Chongqing 400715,China;Chongqing Key Laboratory of Speciality Food Co-Built by Sichuan and Chongqing,Chongqing 400715,China;Key Laboratory of Luminescence Analysis and Molecular Sensing(Southwest University),Ministry of Education,Chongqing 400715,China)
出处
《食品与发酵工业》
CAS
CSCD
北大核心
2024年第3期328-335,共8页
Food and Fermentation Industries
基金
国家自然科学基金青年项目(32201928)
重庆市自然科学基金面上项目(CSTB2022NSCQ-MSX055)
十四五国家重点研发计划项目(2021YFD21001005)。
关键词
铁蛋白
传感元件
策略
食品安全
检测
ferritin
sensing element
strategy
food safety
detection
