摘要
炎症性肠病(inflammatory bowel disease, IBD)是一种慢性肠道疾病,额外营养支持有助于缓解IBD患者病情。研究表明膳食中n-3多不饱和脂肪酸具有良好的抗炎作用,而人群研究表明部分IBD患者额外补充n-3多不饱和脂肪酸无益于其疾病缓解。大规模测序发现n-3多不饱和脂肪酸受体基因FFAR4存在突变,n-3多不饱和脂肪酸在IBD治疗中的不确定结果是否与FFAR4基因的缺失有关,目前尚不清晰。通过对野生型(WT)小鼠和FFAR4基因缺失(FFAR4KO)小鼠进行结肠炎造模,并同时对两组小鼠额外补充n-3多不饱和脂肪酸,实验结果表明,在同时补充n-3多不饱和脂肪酸的情况下,相较于WT小鼠,FFAR4KO小鼠的结肠炎更加严重,表现为更多的体质量丢失、更高的疾病活动指数(disease activity index,DAI)、更高的结肠髓过氧化物酶(MPO)活性以及更高的结肠炎症因子转录水平。该研究为IBD患者得到精确营养干预提供了理论依据。
Inflammatory bowel disease(IBD)is a chronic intestinal inflammatory disease,and additional nutritional support contributes to alleviate IBD symptoms.Studies have shown that dietary n-3 polyunsaturated fatty acids possess anti-inflammatory activity,while clinical trials have reported that additional supplementation with n-3 polyunsaturated fatty acids does not alleviate the disease symptoms in some IBD patients.Large-scale sequencing has revealed mutations in the n-3 polyunsaturated fatty acids receptor gene free fatty acid receptor 4(FFAR4).It remains unclear whether the variable effects of n-3 polyunsaturated fatty acids in IBD treatment are associated with the absence of the FFAR4 gene.In this study,the wild type(WT)and FFAR4 knockout(FFAR4KO)mice were used to establish the colitis models,concurrently supplemented with n-3 polyunsaturated fatty acids. The results showed that compared to those WT mice, the FFAR4KO mice exhibited more severer colitis under simultaneous n-3 polyunsaturated fatty acids supplementation, characterized by greater weight loss, higher disease activity index(DAI), increased colonic myeloperoxidase (MPO) activity, and higher mRNA levels of colonic inflammatory factors. This study provides a theoretical basis for precise nutritional intervention in IBD patients.
作者
张靖伟
姜旋
朱升龙
陈永泉
ZHANG Jingwei;JIANG Xuan;ZHU Shenglong;CHEN Yongquan(School of Food Science and Technology,Jiangnan University,Wuxi 214122,China;Wuxi School of Medicine,Jiangnan University,Wuxi 214122,China)
出处
《食品与生物技术学报》
CAS
CSCD
北大核心
2024年第1期13-19,共7页
Journal of Food Science and Biotechnology
基金
国家自然科学基金项目(31771539,82000808)
无锡市卫生健康委面上基金项目(M202004)。
关键词
炎症性肠病
游离脂肪酸受体4
N-3多不饱和脂肪酸
inflammatory bowel disease
free fatty acid receptor 4
n-3 polyunsaturated fatty acids
