摘要
【目的】研究辣椒内小G蛋白CaROP的生物学功能、蛋白理化性质、蛋白结构及系统发育关系。【方法】运用ProtParam、ProtScale、SignalP5.0、TMHMM、NetPhos3.1和NetCGlyc1.0等生物信息学软件分析9个CaROP蛋白的蛋白理化特性;运用SOPMA和SWISS-MODEL等生物信息学软件预测分析9个CaROP蛋白的结构;运用生物信息学软件MEGA11分析9个CaROP蛋白的系统发育关系。【结果】9个CaROP蛋白的亲水性总平均值均小于0,均为亲水蛋白,其中6个为稳定的亲水蛋白;9个CaROP蛋白均无信号肽,即均为非分泌性蛋白;9个CaROP蛋白无跨膜结构域,即均非膜蛋白;9个CaROP蛋白均存在磷酸化位点,均无糖基化位点。9个CaROP蛋白的主要二级结构原件为无规则卷曲和α-螺旋,其次是β-折叠,最后是β-转角。9个CaROP蛋白聚为4个分支,其中分支Ⅰ和分支Ⅲ各包括1个CaROP蛋白。分支Ⅱ包括2个CaROP蛋白,其余5个CaROP蛋白均聚于分支Ⅳ中。【结论】9个CaROP蛋白具有完整的Rho功能域,均为非分泌性亲水蛋白。9个CaROP蛋白在系统进化树中共聚为了4个分支,其三级结构建模的预测结果也均较为理想,9个CaROP蛋白结构较稳定。
【Objective】To clarify the biological function,physicochemical properties,protein structure and phylogenetic relationship of small G protein CaROP in pepper.【Methods】In this study,the physicochemical properties of nine CaROP proteins were analyzed by using bioinformatics software such as ProtParam,ProtScale,SignalP5.0,TMHMM,NetPhos3.1 and NetCGlyc1.0;The structures of 9 CaROP proteins were predicted and analyzed by using SOPMA,SWISS-MODEL and other bioinformatics software;The phylogenetic relationship of 9 CaROP proteins was analyzed by using bioinformatics software MEGA11.【Results】The results of protein physicochemical properties analysis showed that the total average hydrophilicity of 9 CaROP proteins was less than 0,which were all hydrophilic proteins,and 6 of them were stable hydrophilic proteins;None of the 9 CaROP proteins had signal peptide,namely,they were non secretory proteins;Nine CaROP proteins had no transmembrane domain,namely,none of them were membrane proteins;All 9 CaROP proteins had phosphorylation sites and none had glycosylation sites.The results of protein structure prediction and analysis showed that the main secondary structural elements of 9 CaROP proteins were irregular curls andα-Spiral,followed byβ-Fold,finallyβ-Corner.Phylogenetic analysis showed that nine CaROP proteins were clustered into four branches,of which each of branch I and branch III contained one CaROP protein,branch II contained two CaROP proteins,and the other five CaROP proteins were clustered in branch IV.【Conclusion】Nine CaROP proteins have the Rhoa domain and are all non-secretory hydrophilicproteins.The nine CaROP proteins were clustered into four branches in thephylogenetic tree,and the prediction results of their tertiary structure modeling were also ideal.The structure of the nine CaROP proteins was relatively stable.
作者
马思洁
朱天生
何璐
杨叔青
MA Sijie;ZHU Tiansheng;HE Lu;YANG Shuqing(College of Agriculture,Tarim University,Aral Xinjiang 843300,China;Key Laboratory of Integrated Pest Management of Xinjiang Production and Construction Corps in Southern Xinjiang,Aral Xinjiang 843300,China)
出处
《新疆农业科学》
CAS
CSCD
北大核心
2024年第1期165-175,共11页
Xinjiang Agricultural Sciences
基金
国家自然科学基金项目(32160644)
塔里木大学校长基金项目(TDZKBS202101)
塔里木大学大学生创新训练计划项目(2022039)。
关键词
辣椒
小G蛋白ROP
蛋白理化特性
蛋白结构
系统发育关系
pepper
small G protein ROP
physical and chemical properties of protein
protein structure
phylogenetic relationship
