摘要
为研究禽多杀性巴氏杆菌类兽用生物制品效力评价用菌株,制备了一批禽多杀性巴氏杆菌CVCC44801株,并对菌种的形态、生化特性、培养特性、血清学特性、真空度、纯粹、剩余水分及毒力等进行检定,进一步评估了冻干菌菌数的稳定性,对冻干菌与新鲜培养菌液的毒力进行了比较。试验结果表明,该冻干菌种的形态及生化特性、培养特性、血清学特性、真空度、纯粹、剩余水分、毒力均符合《中华人民共和国兽用生物制品规程》二○○○版菌种标准的规定。冻干菌菌数保持稳定,并且与新鲜培养的攻毒菌液相比,毒力并无差异。本研究为禽多杀性巴氏杆菌类兽用生物制品效力评价用菌株的制备和检定提供参考依据。
In order to study the strains of Pasteurella multocida for the efficacy evaluation of veterinary biological products,a batch of avian Pasteurella mulocida CVCC44801 strains were prepared,and the morphology,biochemical characteristics,culture characteristics,serological characteristics,vacuum degree,purity,residual water and virulence of the strains were tested,and the stability of the freeze-dried bacteria was further evaluated.The virulence of freeze-dried bacteria was compared with that of freshly cultured bacteria.The results showed that the morphology,biochemical characteristics,culture characteristics,serological characteristics,vacuum degree,purity,residual water and virulence of the lyophilized strain were in accordance with the quality standard of the Regulations of the Peoples Republic of China on Veterinary Biological Products 2000 edition.The number of freeze-dried bacteria remained stable,and the virulence was not different from that of freshly cultured bacteria.This study provides a reference for the preparation and verification of strains for the efficacy evaluation of Pasteurella multocida bioproducts for veterinary use.
作者
冯妍
张一帜
王秀丽
王甲
刘燕
姚文生
任小侠
FENG Yan;ZHANG Yi-zhi;WANG Xiu-li;WANG Jia;LIU Yan;YAO Wen-sheng;REN Xiao-xia(China Institute of Veterinary Drug Control,Bejing 100081,China)
出处
《中国兽药杂志》
2024年第1期13-17,共5页
Chinese Journal of Veterinary Drug
基金
中国兽医药品监察所兽药行业公益性重点专项(GY202104)。
