摘要
目的探讨3种中药成分(姜黄素、连翘苷、白藜芦醇)对氧化型低密度脂蛋白(Ox-LDL)诱导的人主动脉内皮细胞(HAEC)损伤的影响。方法实验设空白对照组(等体积基础培养液),溶剂对照组[等体积含二甲基亚砜(DMSO)的基础培养液],Ox-LDL组(20μg/mL Ox-LDL),姜黄素①②③④组(20μg/mL Ox-LDL+2.5μmol/L、5μmol/L、10μmol/L、20μmol/L姜黄素),连翘苷①②③④组(20μg/mL Ox-LDL+10μmol/L、20μmol/L、40μmol/L、80μmol/L连翘苷),白藜芦醇①②③组(20μg/mL Ox-LDL+20μmol/L、40μmol/L、80μmol/L白藜芦醇),采用四甲基偶氮噻唑盐(MTT)法检测细胞活性,并计算细胞增殖率;采用实时荧光定量聚合酶链式反应(qPCR)法检测细胞微管相关蛋白1轻链3(LC3)和环氧合酶2(COX-2)mRNA的表达水平。结果与Ox-LDL组比较,姜黄素、白藜芦醇各剂量组,连翘苷④组细胞增殖率均显著降低(P<0.01或P<0.05);姜黄素③组、连翘苷②③④组、白藜芦醇各剂量组细胞LC3 mRNA表达水平均显著升高,COX-2 mRNA表达水平均显著降低(P<0.01或P<0.05)。结论姜黄素、连翘苷和白藜芦醇可抑制Ox-LDL诱导下HAEC的增殖,可激发受损细胞自噬,抑制炎性因子COX-2表达。
Objective To investigate the effects of three traditional Chinese medicine(TCM)ingredients(curcumin,phillyrin and resveratrol)on the oxidized low-density lipoprotein(Ox-LDL)-induced human aortic endothelial cells(HAECs)injury.Methods HAECs were divided into the blank control group(equal volume of basic culture medium),the solvent control group[equal volume of basic culture medium containing dimethyl sulfoxide(DMSO)],the Ox-LDL group(20μg/mL Ox-LDL),the curcumin groups①②③④(20μg/mL Ox-LDL+2.5μmol/L,5μmol/L,10μmol/L,20μmol/L curcumin),the phillyrin groups①②③④(20μg/mL Ox-LDL+10μmol/L,20μmol/L,40μmol/L,80μmol/L phillyrin),the resveratrol groups①②③(20μg/mL Ox-LDL+20μmol/L,40μmol/L,80μmol/L resveratrol).Cell activity was detected by the methyl thiazolyl tetrazolium(MTT)method,the cell proliferation rate was calculated,the expression levels of microtubule-associated protein 1 light-chain 3(LC3)and cyclooxygenase-2(COX-2)mRNA were detected by the real-time fluorescence quantitative polymerase chain reaction(qPCR).Results Compared with that in the Ox-LDL group,the cell proliferation rate in the phillyrin group④,all the curcumin groups and resveratrol groups significantly decreased(P<0.01 or P<0.05).Compared with those in the Ox-LDL group,the expression levels of LC3 mRNA in the curcumin group③,phillyrin groups②③④and all the resveratrol groups significantly increased,and the expression level of COX-2 mRNA significantly decreased(P<0.01 or P<0.05).Conclusion Curcumin,phillyrin and resveratrol can inhibit the proliferation of HAECs induced by Ox-LDL,stimulate the autophagy of injured cells,and inbibit the expression of inflammatory factor COX-2.
作者
楼彩霞
王弋
代路路
龙淑娴
黄小琼
LOU Caixia;WANG Yi;DAI Lulu;LONG Shuxian;HUANG Xiaoqiong(Guangdong Medical Laboratory Animal Center,Foshan,Guangdong,China 528248;Guangdong Provincial People's Hospital·Guangdong Academy of Medical Sciences,Guangzhou,Guangdong,China 510080)
出处
《中国药业》
CAS
2024年第4期39-42,共4页
China Pharmaceuticals
基金
广东省中医药局面上科研项目[20191046]。
关键词
姜黄素
连翘苷
白藜芦醇
氧化型低密度脂蛋白
人主动脉内皮细胞
细胞自噬
炎性反应
curcumin
phillyrin
resveratrol
oxidized low-density lipoprotein
human aortic endothelial cells
cellular autophagy
inflammatory reaction
