摘要
目的研究猴头菌培养液乙酸乙酯提取物的化学成分。方法通过凝胶柱色谱、硅胶柱色谱、半制备高效液相色谱等技术进行分离和纯化;根据其理化性质数理和波谱对得到的化合物进行结构鉴定。结果从猴头菌培养液乙酸乙酯提取物中分离并鉴定得到22个化合物,分别为3-甲氧基-4-羟基苯甲醛(1),吲哚甲酸甲酯(2),2-呋喃甲酸(3),苯甲酸(4),2(3H)-苯并恶唑酮(5),香草酸(6),没食子酸(7),polisin C(8),bis(2-ethylhexyl)benzene-1,2-dicarboylate(9),p-tyrosyl acetate(4-hydroxyphenethyl acetate)(10),1-(4-hydroxy-3,5-dimethoxyphenyl)ethanon(11),对羟基苯乙酮(12),1-苯基-1,2-丙二醇(13),6-羟基异苯并呋喃-1(3H)-酮(14),对羟基苯乙醛(15),1H-吲哚-3-吡咯甲醛(16),环(脯氨酸-缬氨酸)(17),环(脯氨酸-亮氨酸)(18),环(苯丙氨酸-缬氨酸)(19),环(异亮氨酸-苯丙氨酸)(20),苜蓿素(21),dihydro-4-hydroxy-2(3H)-furanone(22)。结论除化合物16和21外,其他化合物均为首次从猴头菌培养液中分离得到。
Objective To study the chemical constituents of ethyl acetate extracts of the culture solution of Hericium erinaceus.Methods Compounds were isolated and purified using Sephadex LH-20 column chromatography,silica gel column chromatography,semi-preparative HPLC and so on,and their structures were identified based on their physicochemical properties and spectral data.Results A total of 22 compounds were isolated and obtained from the ethyl acetate extracts of the culture solution of Hericium erinaceus,including 3-methoxy-4-hydroxybenzaldehyde(1),methyl indole-3-carboxylate(2),2-furancarboxylic acid(3),benzoic acid(4),2(3H)-benzoxazolone(5),vanillic acid(6),gallic acid(7),polisin C(8),bis(2-ethylhexyl)benzene-1,2-dicarb-oxylate(9),p-tyrosyl acetate(4-hydroxyphenethyl acetate)(10),1-(4-hydroxy-3,5-dimethoxyphenyl)ethanon(11),hydroxyacetophenone(12),1-phenyl-1,2-propanediol(13),6-hydroxy isobenzofuran-1(3H)-one(14),4-hydroxyphenylacetaldehyde(15),1H-indole-3-carbox-aldehyde(16),cyclo(Pro-Val)(17),cyclo(Pro-Leu)(18),cyclo(Phe-Val)(19),cyclo(Ile-Phe)(20),3',5'-dimethoxy-4',5,7-trihydroxyflavone(21),and dihydro-4-hydroxy-2(3H)-furanone(22).Conclusion With the exception of compounds 16 and 21,all other compounds were isolated from the culture solution of Hericium erinaceus for the first time.
作者
姚清颖
张小娟
曾妮
付港
李斌
王炜
YAO Qingying;ZHANG Xiaojuan;ZENG Ni;FU Gang;LI Bin;WANG Wei(School of Pharmacy,Hunan University of Chinese Medicine,Changsha,Hunan 410208,China;Traditional ChineseMedicine and Ethnomedicine Innovation&Development International Laboratory,Hunan University ofChinese Medicine,Changsha,Hunan 410208,China)
出处
《湖南中医药大学学报》
CAS
2024年第2期245-250,共6页
Journal of Hunan University of Chinese Medicine
基金
国家自然科学基金项目(82074122)
湖南中医药大学研究生创新项目(2022CX71)。
关键词
猴头菌培养液
化学成分
小分子化合物
环肽类化合物
生物碱
culture solution of Hericium erinaceus
chemical constituents
small-molecular compounds
cyclopeptides
alkaloids
