摘要
为探究典型抗生素冲击对黄铁矿/硫混合基质(FeS_(2)/S^(0))修复体系反硝化过程的影响,本文以地下水中检出率较高的磺胺类(磺胺甲噁唑(SMZ)和磺胺嘧啶(SDZ))、氟喹诺酮类(恩诺沙星(ENR)和氧氟沙星(OFL))、四环素类(四环素(TCY)和土霉素(OTC))及大环内酯类(红霉素(ERY)和螺旋霉素(SPM))作为典型抗生素,设置其浓度为100ng/L~500μg/L,建立模拟地下水微生态环境实验体系,分别研究其对FeS_(2)/S^(0)修复体系反硝化过程中氮素、可溶性总铁(TFe)、游离Fe^(2+)、硝酸盐还原酶(NAR)、亚硝酸盐还原酶(NIR)与电子传递活性(ETSA)等的变化特征,同时以无抗生素的体系作为空白対照组.结果表明,抗生素对各体系反硝化抑制作用与其种类及浓度密切相关:抑制程度依次为OFL (抑制率5.81%~27.73%)>ENR (3.06%~14.17%)、OTC (1.95%~14.25%)>SMZ (2.54%~11.75%)、SDZ (1.87%~10.90%)>TCY (1.98%~9.44%)、SPM (2.45%~9.43%)>ERY (2.13%~8.47%),抑制率和浓度呈正相关.各实验组中NO_(2)^(-)-N去除速率低于NO_(2)^(-)-N生成速率,NAR和NIR活性均呈现先增后降的单峰变化规律,其酶活峰值均低于空白组;各反应体系中SO_(4)^(2-)-S、TFe与Fe^(2+)浓度与NO_(3)^(-)-N去除率呈正相关,各浓度水平下OFL实验组对反硝化效能抑制最为显著.与空白组相比,各实验体系的ETSA值均有不同程度的下降,尤其是OFL胁迫体系的ETSA值下降幅度最大.硝酸盐还原动力学系数k表明,ng/L水平抗生素各反应体系中的k值差别不大,而μg/L浓度水平时,各反应体系中k值差异明显,OFL反应体系的k值明显低于其他反应体系.
In order to evaluate the performance of denitrification process in the pyrite/sulfur-based remediation system(FeS_(2)/S^(0))under different types of typical antibiotics,sulfonamides(sulfamethoxazole(SMZ)and sulfadiazine(SDZ)),fluoroquinolones(enrofloxacin(ENR)and ofloxacin(OFL)),tetracycline(tetracycline(TCY)and oxytetracycline(OTC)),macrolides(erythromycin(ERY)and spiramycin(SPM))which are usually highly detected in groundwater were taken as typical antibiotics with a set range of concentrations from 100ng/L to 500ug/L.Based on the simulated groundwater microcosm experimental system in this study,the changes in nitrogen,nitrate reductase(NAR),nitrite reductase(NIR)and electron transfer activity(ETSA)during the denitrification in the FeS_(2)/S^(0) remediation system were investigated.The results show that denitrifying inhibition effect was significantly associated with concentration and species of antibiotic at the order of inhibition degree as:OFL(5.81%~27.73%)>ENR(3.06%~14.17%),OTC(1.95%~14.25%)>SMZ(2.54%~11.75%),SDZ(1.87%~10.90%)>TCY(1.98%~9.44%),SPM(2.45%~9.43%)>ERY(2.13%~8.47%),and the higher the concentration,the greater the level of inhibition.In all experimental groups,the removal rate of NO2--N was lower than that of NO^(3)^(-)-N,the activity of both NAR and NIR increased and then decreased,resulting in a single peak change with the greatest reduction.There was a positive correlation of the removal rate of NO^(3)^(-)-N to the concentrations of SO_(4)^(2-)-S,TFe and Fe^(2+)in effluent.The most significant denitrification inhibition occurred in OFL interrupting groups.Contrasting to the control group,the average value of ETSA in each experimental system(especially in the OFL stressing group)decreased.Meanwhile,the k value in each reaction system of antibiotics did not change much at the ng/L level but varied significantly at theμg/L concentration level,and the k value was significantly lower in the OFL reaction system than in other reaction systems.
作者
曹惜霜
信欣
杨雯钰
刘鑫
潘先兵
CAO Xi-shuang;XIN Xin;YANG Wen-yu;LIU Xin;PAN Xian-bing(School of Resources and Environment,Chengdu University of Information Technology,Chengdu 610225,China;Engineering Research Center of Groundwater Pollution Control and Remediation,Ministry of Education,Beijing Normal University,Beijing 100875,China)
出处
《中国环境科学》
EI
CAS
CSCD
北大核心
2024年第2期793-802,共10页
China Environmental Science
基金
国家自然科学基金资助项目(42377083)
地下水污染控制与修复教育部工程研究中心开放基金资助项目资助(GW202211)
四川省大学生创新创业计划项目(202210621041)。
关键词
地下水
硝酸盐
反硝化
抗生素
酶活性
敏感性
groundwater
nitrate
denitrification
antibiotics
enzyme
sensitivity
