摘要
采用同位素内标-超高效相色谱-串联质谱法测定水产品中丁香酚、异丁香酚、甲基丁香酚、甲基异丁香酚、乙酸丁香酚酯、乙酰基异丁香酚和间氨基苯甲酸乙酯甲磺酸盐的测定方法。试样中丁香酚类麻醉剂采用乙腈提取,经HLB固相萃取柱净化,采用超高效相色谱-质谱联用仪进行确认和定量。在1.0~100μg/L的基质匹配曲线,7种麻醉剂在线性范围内良好(R^(2)>0.99)。方法的检出限为0.01~0.03μg/kg,定量限为0.05~0.1μg/kg,加标水平为0.5,10和100μg/kg三水平时,丁香酚类麻醉剂的回收率范围为78.4%~118%,相对标准偏差(n=6)范围为2.5%~8.3%。该方法检测时不仅检测限和定量限低,而且拥有较好的准确性,操作步骤简便,可满足水产品中丁香酚类麻醉剂的残留测定。
A method for the determination of eugenol,isoeugenol,methyl eugenol,methyl isoeugenol,eugenol acetate,acetyl isoeugenol and ethyl m-aminobenzoate mesylate in aquatic products by isotope internal standard ultra-high performance gas chromatography-tandem mass spectrometry(UPLC-MS/MS)was established.The eugenol anesthetic was extracted by acetonitrile,purified by HLB solid phase extraction column,and confirmed and quantified by ultra-high performance phase chromatography-mass spectrometry.The results showed that 7 kinds of eugenol had good linear(R^(2)>0.99)within a covering 1.0-100μg/L concentration range.The detection limits of the method were 0.01-0.03μg/kg,and the limits of quantitation were 0.05-0.1μg/kg.When the spiked levels were 0.5,10 and 100μg/kg,the recovery of eugenol anesthetics ranged from 78.4%to 118%,and the relative standard deviation ranged from 2.5%to 8.3%.The method not only had low limit of detection and limit of quantitation,but also had good accuracy and simple operation procedure,which could satisfy the determination of eugenol anesthetic residues in aquatic products.
作者
陈源
CHEN Yuan(Pu Jia Life Technology(Fuzhou)Co.,Ltd.,Fuzhou 350028)
出处
《食品工业》
CAS
2024年第1期280-283,共4页
The Food Industry
关键词
同位素内标
水产品
丁香酚
超高效相色谱-串联质谱(UPLC-MS/MS)
isotope internal standard
aquatic products
eugenol
ultra-high Performance Liquid Chromatography Tandem Mass Spectrometry(UPLC-MS/MS)
