摘要
目的 研究豨莶草提取物对脑缺血/再灌注大鼠的神经保护作用,并探讨其作用机制。方法 SD大鼠60只,线栓法制备大脑中动脉闭塞(middle cerebral artery occlusion, MCAO)模型,实验分为假手术(Sham)组,模型(MCAO)组,豨莶草低剂量(XXC-L)、中剂量(XXC-M)、高剂量(XXC-H)组,连续给药7天。mNSS评估大鼠神经功能损伤,MRI检测大鼠缺血侧脑梗死体积,Nissl染色检测Nissl阳性细胞数;Western blot检测Bax、Bcl-2、NeuN蛋白表达;免疫荧光染色检测Tunel、NeuN;RT-qPCR检测IL-1β、TNF-α、IL-6 mRNA表达。结果 豨莶草提取物能显著降低MCAO大鼠mNSS评分和缺血侧脑梗死体积(P<0.05,P<0.01),促进缺血侧脑组织Nissl阳性细胞数和NeuN表达、抑制Tunel阳性细胞数(P<0.01);同时,豨莶草提取物能抑制IL-1β、TNF-α、IL-6mRNA及Bax蛋白,促进Bcl-2、NeuN蛋白(P<0.01)。结论 豨莶草提取物对MCAO大鼠具有神经保护作用。
Aim To study the neuroprotective effects of Herba siegesbeckiae extract on cerebral ischemia/reperfusion rats and its mechanism.Methods Sixty SD rats were randomly divided into model group,low,middle and high dose groups of Herba siegesbeckiae,and Sham operation group,and the drug was given continuously for seven days.The degree of neurologic impairment was evaluated by mNSS,and the infarct volume was measured by MRI.The number of Nissl-positive cells was detected by Nissl staining,and the apoptosis was accessed by Tunel staining.Furthermore,the expression of Bax,Bcl-2 and NeuN was observed by Western blot,and the expression of NeuN was detected by immunofluorescence staining.The expression of IL-1β,TNF-αand IL-6 mRNA was performed by RT-qPCR.Results The mNSS score and the volume of ischemic cerebral infarction in the model group were significantly increased,and Herba siegesbeckiae extract treatment significantly decreased the mNSS score and infarct volume(P<0.05,P<0.01).Herba siegesbeckiae extract could increase the number of Nissl-positive cells and the expression of NeuN(P<0.01),and reduce the number of Tunel-positive cells(P<0.01).Western blot showed that Herba siegesbeckiae extract inhibited the expression of Bax,increased Bcl-2 and NeuN in ischemic brain tissue(P<0.01).RT-qPCR showed that Herba siegesbeckiae extract inhibited the expression of IL-1β,TNF-αand IL-6 mRNA in the ischemic brain tissue(P<0.01).Conclusions Herba siegesbeckiae extract can reduce the cerebral infarction volume,improve the neurological function damage,inhibit the apoptosis of nerve cells and the expression of inflammatory factors and promote the expression of NeuN,there by exerting protective effects on MCAO rats.
作者
吴慧玲
吴青青
陈景泉
周彬彬
余正双
杨泽霖
赖文芳
洪桂祝
WU Hui-ling;WU Qing-qing;CHEN Jing-quan;ZHOU Bin-bin;YU Zheng-shuang;YANG Ze-lin;LAI Wen-fang;HONG Gui-zhu(College of Pharmacy,Fujian University of Traditional Chinese Medicine,Fuzhou 350122,China)
出处
《中国药理学通报》
CAS
CSCD
北大核心
2024年第1期70-75,共6页
Chinese Pharmacological Bulletin
基金
国家自然科学基金资助项目(No 82174001,81973503)
福建省自然科学基金项目(No 2021J02014,2020J01727)。
