摘要
本实验以苗龄为90 d的暗紫贝母芽繁苗为实验材料,在组培生根培养过程中添加不同浓度的激素和糖分,研究其对暗紫贝母生根的影响,筛选出暗紫贝母的最适生根培养基。结果表明:在生根培养基为基本培养基(MS)+9.4 g·L^(-1)卡拉胶(car)+0.5 mL·L^(-1)萘乙酸(NAA)+0.5 mL·L^(-1)吲哚丁酸(IBA)+30 g·L^(-1)蔗糖时暗紫贝母生根情况最好,生根率高达81.3%,根系粗壮,且有效根数最多,平均每株有3.4条根系,且没有被污染。本研究为以后建立暗紫贝母组培快繁体系、大规模标准化生产优良暗紫贝母种苗提供参考。
In this study,the 90-day-old buds of Fritilaria unibracteata Hsiao et K.C.Hsia were used as experimental materials,and different concentrations of hormones and sugars were added to study their effects on the rooting of Fritilaria unibracteata Hsiao et K.C.Hsia in tissue culture,and the most suitable rooting medium for Fritilaria unibracteata Hsiao et K.C.Hsia was screened.The results showed that the rooting medium was MS+9.4 g·L~(-1) car+0.5 mL·L~(-1) NAA+0.5 mL·L~(-1) IBA+30 g·L~(-1) sucrose had the best rooting effect,with a rooting rate of 81.3%,a thick root system,and the largest number of effective roots,with an average of 3.4 roots per plant,and no pollution.This study provides a reference for the establishment of a fast propagation system for tissue culture and large-scale standardized production of excellent Fritilaria unibracteata Hsiao et K.C.Hsia seedlings in the future.
作者
字胡润
赵艳娟
管世超
苏娟
文凤
张玉蓉
王晓
李靖瑞
岳健
ZI Hurun;ZHAO Yanjuan;GUAN Shichao;SU Juan;WEN Feng;ZHANG Yurong;WANG Xiao;LI Jingrui;YUE Jian(Yunnan Qinggu Biotechnology Co.,Ltd.,Kunming 650506,Yunnan;Yunnan Shanlihong Biotechnology Co.,Ltd.,Kunming 650229,Yunnan)
出处
《浙江农业科学》
2024年第2期325-328,共4页
Journal of Zhejiang Agricultural Sciences
关键词
暗紫贝母
组织培养
激素
糖分
生根
Fritilaria unibracteata Hsiao et K.C.Hsia
tissue culture
hormones
sugar
rooting
