摘要
为研究微生物源溶菌酶对14种供试菌的体外抑菌活性及对脂多糖(lipopolysaccharide,LPS)诱导的小鼠巨噬细胞RAW264.7细胞炎症的抑制作用,该文采用二倍稀释法测定微生物源溶菌酶对供试菌的最小抑菌浓度(minimum inhibitory concentration,MIC),评价其体外抑菌活性,通过构建LPS诱导的小鼠巨噬细胞RAW264.7炎症模型,以细胞中一氧化氮(nitric oxide,NO)及肿瘤坏死因子-α(tumor necrosis factor-α,TNF-α)的分泌量为指标,评价微生物源溶菌酶的抗炎活性。结果表明,微生物源溶菌酶对大肠杆菌、枯草芽孢杆菌、金黄色葡萄球菌、沙门氏菌、单增李斯特菌、耐热芽孢杆菌、酿酒酵母、酪丁酸梭菌、产黄青霉、黑曲霉和根霉11种菌均表现出良好的抑菌效果,具有较强的广谱抑菌性;在试验浓度范围内,对保加利亚乳杆菌、嗜热链球菌、乳酸乳球菌乳亚种3种益生菌无抑制效果,反而具有生长促进效果。微生物源溶菌酶对LPS诱导的小鼠巨噬细胞RAW264.7炎症模型表现出明显的抗炎作用,可显著降低NO及TNF-α的分泌,且与微生物源溶菌酶的作用浓度呈正相关。在试验浓度范围内,与模型组相比NO分泌量最高可降低57.92%,TNF-α分泌量最高可降低36.75%。微生物源溶菌酶具有良好的抑菌和抗炎活性,为其作为食品添加剂在抑制腐败菌生长、促进益生菌增殖等方面应用提供了理论基础。
The in vitro inhibitory activities of microbial lysozyme against 14 microbial species and lipopolysaccharide(LPS)-induced inflammation in RAW264.7 macrophages were determined.The minimum inhibitory concentration(MIC)of the microbial lysozyme against the microbial species were determined by double dilution method,on the basis of which the in vitro antimicrobial activity of the lysozyme was measured.RAW264.7 macrophages were used to establish he LPS-induced inflammation model,and the anti-inflammatory activity of microbial lysozyme was evaluated based on the secretion of nitric oxide(NO)and tumor necrosis factor-alpha(TNF-α)in cells.The results showed that the microbial lysozyme exerted inhibitory effects against Escherichia coli,Bacillus subtilis,Staphylococcus aureus,Salmonella sp.,Listeria monocytogenes,Bacillus thermophilus,Sac⁃charomyces cerevisiae,Clostridium butyricum,Penicillium flavum,Aspergillus niger,and Rhizopu sp.,demonstrating strong broad-spectrum antimicrobial activities.Within the tested concentration range,the microbial lysozyme had no inhibitory effect against Lactobacillus bulgaricus,Streptococcus thermophilus or Lactococcus lac⁃tis lactis,but promoted their growth.The microbial lysozyme showed significant inhibitory effect on LPS-induced inflammation of RAW264.7 macrophages,which significantly reduced the secretion of NO and TNF-αin a concentration-dependent manner.Compared with the model group,the microbial lysozyme reduced the secretion of NO and TNF-αby 57.92%and 36.75%,respectively.Microbial lysozyme has good antimicrobial and anti-inflammatory activities,which provides a theoretical basis for its application as a food additive in inhibiting the growth of spoilage microorganisms and promoting the proliferation of probiotics.
作者
司奇
胡雨
戴静
王珍珍
沙如意
毛建卫
SI Qi;HU Yu;DAI Jing;WANG Zhenzhen;SHA Ruyi;MAO Jianwei(School of Biological and Chemical Engineering,Zhejiang Collaborative Innovation Center for Agricultural Bioresources and Biochemical Manufacturing,Zhejiang Key Laboratory of Agricultural Products Chemistry and Bioprocessing Technology,Zhejiang University of Science and Technology,Hangzhou 310023,Zhejiang,China)
出处
《食品研究与开发》
CAS
2024年第4期68-73,88,共7页
Food Research and Development
基金
浙江省重点研发计划项目(2017C02009)。
关键词
微生物源溶菌酶
最小抑菌浓度
抑菌活性
小鼠巨噬细胞
抗炎活性
microbial lysozyme
minimum inhibitory concentration
antimicrobial activity
mouse macrophages
anti-inflammatory activity
