摘要
为优化小春花木犀草素提取工艺,并探讨其抗氧化活性。以小春花为试材,采用单因素和正交试验对小春花中天然产物木犀草素的提取工艺进行优化,得到最佳提取条件,并通过测定小春花提取物对2,2-联氮-二(3-乙基-苯并噻唑-6-磺酸)二铵盐(ABTS+)自由基、1,1-二苯基-2-三硝基苯肼自由基(DPPH·)、羟基自由基(·OH)的清除能力及总还原力,研究其抗氧化活性。结果表明,最佳提取工艺条件为:超声时间25 min,液料比25∶1(mL/g),乙醇体积分数60%,超声温度85℃,通过验证试验得到该条件下木犀草素提取量为4.88 mg/g。抗氧化试验结果表明,小春花提取物对DPPH·、·OH和ABTS+自由基清除率的IC50分别为14.9、78.6、80.2 mg/L,是一种潜在的天然抗氧化剂。优化的小春花中木犀草素提取工艺合理可行,且提取物具有较强的抗氧化活性。
To optimize the extraction process of luteolin from Botrychium ternatum and to investigate its antioxidant activity.In this study,single factors and orthogonal experiments were used to optimize the extraction process of luteolin from Botrychium ternatum,and the optimal extraction conditions were obtained.The scavenging abilities on free radicals including 2,2-diazo-bis(3-ethyl-benzothiazole-6-sulfonic acid)diamiammonium salt(ABTS+),1,1-diphenyl-2-trinitrophenylhydrazine(DPPH),hydroxyl free radical(·OH)and total reducing power of the extract were determined to investigate the antioxidant activities.The results showed that the optimal extraction conditions were determined as follows:ultrasonic time of 25 min,liquid-solid ratio of 25∶1(mL/g),ethanol volume fraction of 60%,and ultrasonic temperature of 85℃.The extraction amount of luteolin was 4.88 mg/g under these conditions.The results of antioxidant tests showed that the IC50 values of DPPH·,·OH and ABTS+free radical scavenging rates of the extract were 14.9,78.6 and 80.2 mg/L,respectively,indicating that the extract could be a potential natural antioxidant.This study provided theoretical basis for the development of the resources of Botrychium ternatum.The optimized extraction process of luteolin from Botrychium ternatum is reasonable and feasible,and the extract has superior antioxidant activity.
作者
何若男
陈欢
易超
林启凰
张豫杰
吴婷
陈仲巍
HE Ruonan;CHEN Huan;YI Chao;LIN Qihuang;ZHANG Yujie;WU Ting;CHEN Zhongwei(Department of Pharmacy,Xiamen Medical College,Xiamen 361023,China)
出处
《保鲜与加工》
CAS
北大核心
2024年第2期23-30,共8页
Storage and Process
基金
2019年福建省中青年教师教育科研项目(JAT190852)
厦门医学院大学生创新创业训练计划项目(202312631067
202212631045)
福建省级大学生创新创业训练计划项目(S202112631022)。
关键词
小春花
木犀草素
超声辅助
提取工艺
抗氧化活性
Botrychium ternatum
luteolin
ultrasonic assistance
extraction technology
antioxidant activity
