摘要
目的观察电针下合穴“足三里”“上巨虚”对慢传输型便秘(STC)大鼠肠道运动及促炎性因子表达的影响。方法24只健康清洁级SD大鼠随机分为空白组、模型组、药物组和电针组,每组各6只。采用复方地芬诺酯混悬液10 mg·kg^(-1)剂量灌胃制备STC大鼠模型,每天1次,连续14 d。空白组予等剂量0.9%氯化钠溶液灌胃。电针组给予双侧“足三里”“上巨虚”电针治疗,选择疏密波,频率2/50 Hz,电流大小以局部肌肉轻微抖动为度,每次治疗20 min,每天1次,连续治疗14 d。药物组予枸橼酸莫沙必利片混悬液按照1.37 mg·kg^(-1)、1 mL·100 g^(-1)剂量灌胃治疗,疗程同上。观察各组治疗后首粒黑便排出时间、小肠推进率,采用HE染色观察结肠组织病理形态学变化,采用Western-blot法检测肠道肿瘤坏死因子α(TNF-α)、白介素1β(IL-1β)、CD68蛋白表达。结果模型组大鼠首粒黑便排出时间较空白组显著加长,小肠推进率显著降低(P<0.05);药物组和电针组大鼠经治疗后,与模型组比较,首粒黑便排出时间显著减少、小肠推进率显著提高(P<0.05)。HE染色可见造模后大鼠结肠黏膜组织出血,组织形态学结构破坏,伴上皮细胞变性坏死及炎性细胞浸润;电针和药物治疗能够减少炎性细胞浸润,改善组织形态学变化。Western-blot结果显示,模型组大鼠TNF-α、IL-1β、CD68蛋白表达较空白组显著升高(P<0.05);药物组和电针组大鼠经治疗后,结肠组织TNF-α、IL-1β、CD68蛋白表达较模型组显著降低(P<0.05)。结论电针治疗能够改善STC大鼠肠道运动功能,修复结肠组织病理形态学改变,其机制可能与下调TNF-α、IL-1β、CD68蛋白表达,抑制炎性反应有关。
Objective To observe the effect of electroacupuncture at"Zusanli"(ST 36)and"Shangjuxu"(ST 37)on intestinal motility and proinflammatory factor expression in rats with slow transit constipation(STC).Methods 24 healthy and clean SD rats were randomly divided into the blank group,the model group,the drug group and the electroacupuncture group,with 6 rats in each group.STC rat models were prepared by gavage of compound diphenoxylate suspension at 10mg·kg^(-1),once a day,for 14 days.The blank group was given 0.9% sodium chloride solution at the same dose by gavage.The electroacupuncture group received bilateral electroacupuncture treatment for"Zusanli"and"Shangjuxu"points,and selected dense-sparse waves with the frequency of 2/50 Hz,and the current was measured by slight local muscle twitch;Each treatment lasted for 20 min,once a day,for 14 consecutive days.The drug group was treated with suspension of Mosapride citrate tablets by gavage at doses of 1.37 mg·kg^(-1)and 1 mL·100 g^(-1),and the treatment course was the same as above.The first black stool excretion time and small intestinal propulsion rate were observed in each group after treatment.The pathological and morphological changes of colon tissue were observed by hematoxylin and eosin(HE)staining,and the expressions of tumor necrosis factor-α(TNF-α),interleukin-1β(IL-1β)and CD68 in intestinal tissue were detected by Western-blot.Results The first black stool excretion time in the model group was significantly longer than that in the blank group,and small intestine propulsion rate was significantly decreased(P<0.05).After treatment,compared with the model group,the time of first black stool excretion in the drug group and the electroacupuncture group was significantly reduced,and the small intestine propulsion rate was significantly increased(P<0.05).HE staining showed bleeding in the colonic mucosal tissue of rats after modeling,and damaged histomorphological structure,accompanied by epithelial cell degeneration and necrosis and inflammatory cell infiltration.Electroacupuncture and drug therapy could reduce inflammatory cell infiltration and improve histomorphological changes.Western-blot results showed that the expressions of TNF-α,IL-1β and CD68 in the model group were significantly increased compared with those in the blank group(P<0.05).After treatment,the expressions of TNF-α,IL-1β and CD68 in colon tissue of rats in the drug group and the electroacupuncture group were significantly decreased compared with those in the model group(P<0.05).Conclusion Electroacupuncture can improve the intestinal motional function and repair the pathomorphological changes of colonic tissue in STC rats,and its mechanism may be related to down-regulating the expression of TNF-α,IL-1β and CD68 protein and inhibiting the inflammatory response.
作者
刘跟莉
李晓宁
师帅
李艳
管淑敏
付瑶
LIU Genli;LI Xiaoning;SHI Shuai;LI Yan;GUAN Shumin;FU Yao(Heilongjiang University of Chinese Medicine,Harbin 150040,China;Department of Acupuncture and Moxibustion,the Second Affiliated Hospital of Heilongjiang University of Chinese Medicine,Harbin 150001,China)
出处
《长春中医药大学学报》
2024年第2期167-173,共7页
Journal of Changchun University of Chinese Medicine
基金
黑龙江中医药大学孙申田青年科学基金(2021KY-03)
黑龙江省中医药科研项目(ZHY2023-158)。
关键词
电针
慢传输型便秘
肠道运动
炎性反应
electroacupuncture
slow transit constipation
intestinal movement
inflammatory reaction
