摘要
为了解运旱系列小麦品种(系)品质相关功能基因组成,利用与高分子量麦谷蛋白亚基、籽粒硬度以及小麦粉色泽等品质性状相关的14个高通量KASP标记对24份运旱小麦进行检测。结果表明,全部供试材料均未检测出1RS/1BL和1RS/1AL易位系;糯性基因Wx-B1的突变型Wx-B1b占比8.33%;小麦粉色泽相关氧化酶基因优异等位变异Ppo-A1b占比12.5%、Ppo-D1a占比16.67%、Lox-B1a占比91.67%以及TaPod-A1b占比为4.17%;籽粒硬度基因Pinb-D1的突变型Pinb-D1b占比91.67%,Pinb-B2的突变型Pinb-B2b占比25%,未检测到Pina-D1的突变型Pina-D1b;在Glu-A1位点含有高分子量麦谷蛋白优质亚基基因的材料占比83.33%、Glu-B1位点含有优质亚基基因的材料占比4.17%、Glu-D1位点含有优质亚基基因的材料占比33.33%。研究明确了运旱系列小麦品种(系)品质功能基因的组成,为山西省小麦品种的品质改良提供了参考。
In order to understand the composition of functional genes of the quality,high-throughput kompetitive allele specific PCR(KASP)assay related to high-molecular weight glutenin subunits,grain hardness and flour color was used to detect 24 Yunhan series wheat cultivars(lines).The results indicated that 1RS/1BL and 1RS/1AL translocation line was not detected in all tested cultivars(lines).Mutant Wx-B1b of waxy gene Wx-B1 accounted for 8.33%.Elite alleles Ppo-A1b of oxidase gene for flour color accounted for 12.5%,Ppo-D1a accounted for 16.67%,Lox-B1a accounted for 91.67%and TaPod-A1b accounted for 4.17%.The mutant Pinb-D1b of the grain hardness gene Pinb-D1 accounted for 91.67%,the mutant Pinb-B2b of Pinb-B2 accounted for 25%,and the mutant Pina-D1b of Pina-D1 was not detected.The proportion of wheat materials with gene of favorable high molecular weight glutenin subunit at Glu-A1 site was 83.33%,the proportion of wheat materials with gene of favorable high molecular weight glutenin subunit at Glu-B1 site was 4.17%,and the proportion of wheat materials with gene of favorable high molecular weight glutenin subunit at Glu-D1 site was 33.33%.This study clarified the composition of quality functional genes of Yunhan series wheat varieties(lines),and provided reference for quality improvement of wheat varieties in Shanxi province.
作者
王倩
任文斌
赵智勇
Wang Qian;Ren Wenbin;Zhao Zhiyong(Institute of Cotton Research,Shanxi Agricultural University,Yuncheng 044000)
出处
《中国粮油学报》
CAS
CSCD
北大核心
2024年第1期47-53,共7页
Journal of the Chinese Cereals and Oils Association
基金
山西省重点研发计划项目(202102140601001)。
