摘要
研究以前期筛选的长牡蛎(Crassostrea gigas)弧菌抗性家系为亲本,构建40个全同胞家系,评估了弧菌抗性选育家系生长和存活性状的遗传参数及多个环境下的基因型与环境互作。结果显示,在不同日龄下,壳高和壳长的遗传力分别为(0.46±0.10)—(0.59±0.13)和(0.30±0.08)—(0.59±0.13),为中高等遗传力,存活性状的遗传力为(0.25±0.06)—(0.41±0.11),为中低等遗传力。不同日龄的壳高和壳长遗传相关(0.429—0.828)均大于表型相关(0.192—0.611),除180日龄时,遗传和表型相关为低度正相关,其余各阶段均为中高度正相关;生长与存活性状的遗传和表型相关分别为(–0.138—0.380)和(–0.073—0.512),表现为中低度相关性。研究中生长和存活性状在两个环境间的遗传相关分别为(0.34—0.68)和(0.32—0.50),均小于0.7,表明乳山和荣成海区家系的生长和存活性状间存在明显的基因型与环境互作效应,因此对长牡蛎弧菌抗性进行选育时需要针对不同的养殖环境制定不同的育种方案。研究为长牡蛎抗弧菌品系的选育提供基础资料和科学依据。
The Pacific oyster(Crassostrea gigas)is an important aquaculture shellfish,which has been widely cultured around the world.In recent years,C.gigas has experienced varying degrees of mass mortality during summer in different breeding areas globally.Our previous study revealed a close relationship between the pathogenic Vibrio alginolyticus and the massive summer mortality of farmed oysters in northern China.In this study,we estimated the genetic parameters of growth and survival traits in C.gigas families that demonstrated resistance to V.alginolyticus through selective breeding.The G1 generation resistant families of C.gigas were developed by selecting individuals with resistance to V.alginolyticus infection.A total of 40 G2 families were established by crossing one female with one male selected from G1 resistant families.Univariate animal model and paternal and maternal threshold animal model were used to estimate the genetic parameters of growth and survival traits at different ages of 120,180,300,400,and 460 days for culture.The results showed that in the Rongcheng area,the heritability of shell height ranged from 0.46±0.10(460d)to 0.53±0.11(120d),indicating a moderate to high level of heritability.Similarly,the heritability of shell length ranged from 0.30±0.08(120d)to 0.56±0.11(180d)in the Rongcheng area,also indicating a moderate to high level of heritability.In the Rushan area,the heritability of shell height ranged from 0.55±0.14(460d)to 0.59±0.13(300d),indicating a high level of heritability.Likewise,the heritability of shell length ranged from 0.50±0.12(300d)to 0.59±0.13(400d)in the same area,also indicating a high level of heritability.In the Rongcheng area,the heritability of survival traits ranged from 0.25±0.06(180d)to 0.41±0.09(460d).Similarly,in the Rushan area,the heritability of survival traits ranged from 0.27±0.07(300d)to 0.41±0.11(460d).Overall,both marine areas exhibit a heritability at a low to moderate level.The correlation analysis of growth and survival traits showed that the genetic correlation(ranged from 0.429 to 0.828)between shell height and shell length at different ages was greater than the phenotypic correlation(ranged from 0.192 to 0.611).Moreover,except for 180 days,the genetic and phenotypic correlation between shell height and shell length exhibited a low positive correlation,while at all other stages,they showed a moderate to high positive correlation.The genetic and phenotypic correlations of growth and survival traits ranged from–0.138 to 0.380 and–0.073 to 0.512,respectively.Additionally,except for 120d,the genetic correlation and phenotypic correlations between growth and survival traits demonstrated a positive correlation.In the Rongcheng area,excluding 300d,the phenotypic correlations between growth and survival traits were low.In contrast,there was a low positive correlation between growth and survival traits at all stages in the Rushan area.The genotype-environment interaction effects of growth and survival traits were estimated by culturing oysters in Rushan and Rongcheng aquaculture areas at different ages.The results showed that the genetic correlation of shell height traits in Rushan and Rongcheng aquaculture areas was 0.34±0.07 to 0.68±0.01.For the shell length traits,the genetic correlation in two areas ranged from 0.35±0.06 to 0.63±0.03.For the survival traits,the genetic correlation in two areas ranged from 0.32±0.08 to 0.50±0.03.The growth and survival traits of Vibrio-resistant oysters at different ages was less than 0.7,indicating a significant genotype-environment interaction effect between the growth and survival traits in the Rushan and Rongcheng aquaculture areas.Therefore,different breeding plans should be developed for different culture environments when performing selection breeding of Vibrio-resistant strains of C.gigas.This study provides fundamental information to support the breeding of Vibrio-resistant strains of C.gigas.
作者
李寅
鲁林基
杨奔
李鹏飞
徐成勋
李琪
刘士凯
LI Yin;LU Lin-Ji;YANG Ben;LI Peng-Fei;XU Cheng-Xun;LI Qi;LIU Shi-Kai(Key Laboratory of Mariculture,Ministry of Education,Ocean University of China,Qingdao 226100,China)
出处
《水生生物学报》
CAS
CSCD
2024年第4期673-682,共10页
Acta Hydrobiologica Sinica
基金
国家重点研发计划(2022YFD2400300)
山东省重点研发计划服务山东项目(2021ZLGX03)资助。
关键词
溶藻弧菌
遗传力
遗传相关
基因型与环境互作效应
选育
长牡蛎
Vibrio alginolyticus
Heritability
Genetic correlations
Genotype by environment interaction
Breeding
Crassostrea gigas
