摘要
宣纸是我国传统手工纸的重要门类,更是古籍书画文物的重要载体,对中华文化的流传起着重要的作用。然而现存的宣纸文物面临着各种病害的侵袭,微生物侵害和纸张酸化就是其中的主要威胁之一。本研究用一批清末民初的手工纸样品制备混合菌液,通过回植培养的方式研究了pH4.01、pH7.00、pH10.00条件下微生物群落结构动态和对宣纸的侵蚀。结果显示,不同pH处理会显著影响宣纸中细菌的群落组成,随着pH的升高,样本中细菌的群落多样性更加丰富。不同pH下真菌的群落结构则没有明显的聚类关系。同时,环境因子关联分析的结果显示细菌群落结构变化与纸张表面pH相关性高,而真菌群落变化与纸张重量变化相关性高。纤维素降解与细菌和真菌群落的相关性相差不大。
Traditional Chinese handmade paper is an important carrier of the ancient relics of books,paintings and calligraphies,which plays an essential role in the spread of Chinese culture.At present,these paper relics are facing great threats,including microbial damage and paper acidification.In this study,the effects of different pH treatments on the process of microbial erosion of Xuan paper,a type of traditional Chinese handmade paper,were studied by inoculating Xuan paper with mixed suspension of microbes isolated from some old paper samples from the late Qing Dynasty and the Early Republican,and were cultured under pH4.01,pH7.00,pH10.00 treatment.The results showed that pH significantly affected the bacterial community composition on Xuan paper.As pH increased,the bacterial community in the samples became more diverse.No such effect was seen on the fungal diversity.The correlation analysis showed that the change of bacterial community structure was more correlated with the surface pH,whereas the change of fungal community was more correlated with the change of paper weight.Cellulose degradation of paper was correlated with both bacteria and fungi similarly.
作者
史砚
杨诗逸
钟江
SHI Yan;YANG Shiyi;ZHONG Jiang(Department of Microbiology and Immunology,School of Life Sciences,Fudan University,Shanghai 200433,China)
出处
《复旦学报（自然科学版）》
CAS
CSCD
北大核心
2024年第1期77-86,共10页
Journal of Fudan University：Natural Science
基金
国家重点研发计划(2019YFC1520402)。
关键词
微生物群落多样性
PH
高通量测序
纸质文物保护
microbial community diversity
pH
high-throughput sequencing
conservation of paper relics
