摘要
目的 探讨标本手动颠倒混匀次数对红细胞沉降率(ESR)的影响。方法 收集200例受试者的ESR标本检测前手动颠倒混匀3、6、9、12和15次,收集每组的ESR结果,以标本手动颠倒混匀12次为参考标准,用R(4.2.1)软件进行数据分析,使用Shapiro-Wilk进行正态分布检验,多组间比较采用Friedman检验,以及采用Spearman相关性分析不同手动颠倒混匀次数和ESR之间的相关性。结果 200例受试者标本分析前按照标本手动颠倒混匀次数依次分为手动颠倒混匀3、6、9、12、15次5个组,每组ESR中位数依次为13.0、11.5、11.0、8.0、11.0 mm/h。Friedman检验显示,5个组ESR结果比较差异有统计学意义(P<0.05)。手动颠倒混匀3、6次与手动颠倒混匀12次ESR结果比较差异有统计学意义(P<0.05)。Spearman相关性分析结果显示,标本手动颠倒混匀次数与ESR之间呈负相关(r=-1.000,P=0.017)。结论 标本手动颠倒混匀9次可达到充分混匀的状态,手动颠倒混匀次数过少会导致ESR偏高,临床实验室应重视标本前处理过程,确保整个检验过程的质量。
Objective To investigate the effect of manually reverse mixing times on erythrocyte sedimentation rate(ESR).Methods The ESR samples of 200 subjects were manually mixed for 3,6,9,12 and 15 times before detection.The ESR results of each group were collected,and the samples were manually mixed for 12 times as the reference standard.R(4.2.1)software was used for data analysis,Shapiro-Wilk was used for normal distribution test,Friedman test was used for multi-group comparison,and Spearman correlation analysis was used to analyze the correlation between different manually reverse mixing times and ESR.Results Before specimen analysis,200 subjects were divided into 5 groups according to the manually reverse mixing for 3,6,9,12 and 15 times.The median ESR of each group was 13.0,11.5,11.0,8.0 and 11.0 mm/h in turn.Friedman test showed that there were significant differences in ESR results among the 5 groups(P<0.05).There were significant differences in ESR results between manually reverse mixing for 3 and 6 times and manually reverse mixing for 12 times(P<0.05).Spearman correlation analysis showed that there was a negative correlation between the manually reverse mixing times and ESR(r=-1.000,P=0.017).Conclusion Specimens can be fully mixed by manually reverse mixing for 9 times.Too few times of manually reverse mixing can lead to high ESR.Clinical laboratories should pay attention to the pre-treatment of specimens to ensure the quality of the whole test process.
作者
李畅
哈斯朝鲁
李欣
郭雅静
李凌
LI Chang;Hasichaolu;LI Xin;GUO Yajing;LI Ling(Department of Medical Laboratory Medicine,First Hospital of Shanxi Medical University,Taiyuan,Shanxi 030001,China)
出处
《国际检验医学杂志》
CAS
2024年第4期476-479,共4页
International Journal of Laboratory Medicine
基金
2022年度山西省科技战略研究专项一般项目(202204031401143)。
关键词
红细胞沉降率
手动颠倒混匀次数
实验室
erythrocyte sedimentation rate
manually reverse mixing times
laboratory
