摘要
目的探讨铁代谢在有氧运动改善动脉粥样硬化(AS)小鼠心肌细胞凋亡中的作用机制。方法选取8周龄雄性ApoE^(-/-)基因敲除小鼠,采用随机数字表法将其分为对照组、模型组及有氧运动组,每组9只小鼠。模型组及有氧运动组小鼠均给予"西方类型"膳食饲料喂养12周以制作AS动物模型,有氧运动组小鼠在制模期间同时进行有氧运动干预,持续训练12周,对照组小鼠则给予正常饲料喂养。于训练12周后采用TUNEL染色法检测各组小鼠心肌细胞凋亡情况;采用免疫组化技术检测各组小鼠心肌组织中铁蛋白重链1(FTH1)、谷胱甘肽过氧化物酶4(GPX4)表达;采用Western blot技术检测各组小鼠心肌组织中FTH1、GPX4蛋白水平;采用普鲁士蓝染色检测各组小鼠心肌组织中铁沉积情况;通过试剂盒检测各组小鼠心肌组织中铁、丙二醛(MDA)及谷胱甘肽过氧化物酶(GSH-PX)含量。结果TUNEL染色结果显示模型组出现明显心肌细胞凋亡,有氧运动组心肌凋亡情况较模型组明显减轻。普鲁士蓝染色显示模型组心肌出现明显铁沉积现象,而有氧运动组铁沉积情况较模型组明显减轻。免疫组化检测显示模型组FTH1及GPX4表达量均较对照组降低,有氧运动组上述指标表达量均较模型组明显升高。Western blot检测显示有氧运动组FTH1、GPX4蛋白水平均较模型组明显升高,且心肌组织中的铁及MDA含量明显低于模型组,GSH-PX含量显著高于模型组。结论有氧运动能显著改善AS小鼠心肌细胞凋亡,其作用机制可能与有氧运动改善心肌组织铁代谢、降低氧化应激水平及抑制铁超载有关。
Objective To demonstrate any role of iron metabolism in the inhibition by aerobic exercise of myocardial apoptosis in atherosclerotic mice.Methods Eight-week-old male ApoE^(-/-)gene knockout mice were randomly divided into a control group,a model group and an aerobic exercise group,each of 9.A model of atherosclerosis was induced in the rats of the model and aerobic exercise groups by feeding them a"western"diet for 12 weeks.During that time the aerobic exercise group only was given aerobic exercise training.The control group was fed normal rat chow during that period.Myocardial apoptosis was detected using TUNEL staining,and the expression and localization of ferritin heavy chain 1(FTH1)and glutathione peroxidase 4(GPX4)in the myocardium used immunohistochemistry.Western blotting was applied to detect the FTH1 and GPX4 protein levels,and iron deposition in the myocardium was detected using Prussian blue staining.Iron,lipid peroxide malondialdehyde(MDA)and glutathione peroxidase(GSH-PX)in the myocardial tissue were also measured.Results The TUNEL staining showed significant apoptosis in the model group.In the aerobic exercise group it was significantly less.There was obvious iron deposition in the myocardia of the model group,which was significantly reduced in the aerobic exercise group.The average FTH1 and GPX4 levels in the model group were lower than in the control group,and significantly elevated in the aerobic exercise group.in the aerobic exercise group than in the model group.Iron and MDA levels in the aerobic exercise group were significantly lower,on average,than among the model group,while that of GSH-PX was significantly higher.Conclusions Aerobic exercise can significantly inhibit cardiomyocyte apoptosis in atherosclerotic mice.The mechanism may be closely related to better iron metabolism,reduced oxidative stress and the inhibition of iron overload.
作者
刘西花
马甜甜
秦芳
于子夫
高杰
Liu Xihua;Ma Tiantian;Qin Fang;Yu Zifu;Gao Jie(The Affiliated Hospital of Shandong University of Traditional Chinese Medicine,Jinan 250014,China;School of Rehabilitation Medicine,Shandong University of Traditional Chinese Medicine,Jinan 250355,China;Maternal and Child Health Development Research Center of Shandong Maternal and Child Health Hospital,Jinan 250014,China)
出处
《中华物理医学与康复杂志》
CAS
CSCD
北大核心
2024年第1期7-12,共6页
Chinese Journal of Physical Medicine and Rehabilitation
基金
国家自然科学基金青年基金项目(81802239)。
关键词
有氧运动
动脉粥样硬化
心肌组织
铁代谢
凋亡
Aerobic exercise
Atherosclerosis
Myocardium
Iron metabolism
Apoptosis
