摘要
为了解秦皇岛昌黎县水产养殖海域微生物分布情况及各种抗性基因的污染状况,使用高通量测序技术,采用16S rDNA基因测序和宏基因组测序方法对春季养殖海域中的海水、底泥及土著鱼类矛尾复鰕虎鱼肠道内容物进行研究.结果表明,海水中以变形菌门(Proteobacteria)、厚壁菌门(Firmicutes)和拟杆菌门(Bacteroidota)为主要优势菌门;底泥中以变形菌门(Proteobacteria)、泉古菌门(Crenarchaeota)、酸杆菌门(Acidobacteriota)和放线菌门(Actinobacteriota)为主;鰕虎鱼肠道内容物以变形菌门(Proteobacteria)、蓝藻细菌(Cyanobacteria)、厚壁菌门(Firmicutes)和拟杆菌门(Bacteroidota)为主.底泥样本中的微生物多样性最丰富,其次是海水样本,鱼类肠道内容物的微生物多样性最低.且不同位点的同类样本之间微生物多样性较为相似,同一位点的不同类样本中的微生物多样性差别较大.对于不同位点的样本,各位点海水样本之间微生物群落结构均存在显著差异,各位点底泥样本之间差异较小,且有些底泥样本组间不存在显著的微生物组成差异.在所有样本组中,检测到的抗性基因主要为:β-内酰胺类抗生素抗性基因有5个(bla_(OXA-325)、cepS、bla_(CARB-20)、bla_(OXA-55)和bla_(TRU-1)),氨基糖苷类抗生素抗性基因有4个[aac(6′)-IIb、amrA、aac(6′)-Ie-aph(2″)-Ia和aph(3′)-Vc].抗生素抗性基因与微生物群落之间也存在一定的相关性.
In order to understand the distribution of microorganisms and various antibiotic resistance genes in the aquaculture area of Changli County,Qinhuangdao,high-throughput sequencing technology was used in this study.We utilized 16S rDNA gene sequencing and metagenome sequencing methods to analyze the seawater,sediment,and gut contents of the local fish Synechogobius hasta in the aquaculture area in spring.The results showed that Proteobacteria,Firmicutes,and Bacteroidota were the dominant bacteria in seawater;and Proteobacteria,Crenarchaeota,Acidobacter,and Actinobaciota were rich in the sediment;whereas Proteobacteria,Cyanobacteria,Firmicutes,and Bacteroidota were in relatively high abundance in fish gut contents.The microbial diversity of sediment samples was the most abundant,followed by seawater samples,and the microbial diversity of fish intestinal contents was the lowest.Moreover,the microbial diversity of similar samples was relatively similar,and the microbial diversity of different types of samples was quite different.For samples at different sites,there were significant differences between seawater samples at each site,and there were small differences between sediment samples at each site,and some sediment sample groups did not have significant differences in microbial composition.In all sample groups,five β-lactam antibiotic resistance genes(bla_(OXA-325),cepS,bla_(CARB-20),bla_(OXA-55),and bla_(TRU-1))and four aminoglycoside antibiotic resistance genes[aac(6′)-IIb,amrA,aac(6′)-Ie⁃aph(2″)-Ia,and aph(3′)-Vc]were detected.There was also a certain correlation between antibiotic resistance genes and microbial communities.
作者
王秋水
程波
刘悦
邓婕
徐岩
孙朝徽
袁立艳
左嘉
司飞
高丽娟
WANG Qiu-shui;CHENG Bo;LIU Yue;DENG Jie;XU Yan;SUN Chao-hui;YUAN Li-yan;ZUO Jia;SI Fei;GAO Li-juan(Institute of Analysis and Testing,Beijing Academy of Science and Technology(Beijing Center for Physical and Chemical Analysis),Beijing 100089,China;Key Laboratory of Aquatic Product Quality and Safety Control,Ministry of Agriculture and Rural Affairs,Chinese Academy of Fishery Sciences,Beijing 100141,China;Beidaihe Central Experimental Station,Chinese Academy of Fishery Sciences,Qinhuangdao 066100,China)
出处
《环境科学》
EI
CAS
CSCD
北大核心
2024年第1期567-575,共9页
Environmental Science
基金
世界自然基金会(WWF)蔚蓝星球基金项目(PORO001427)
北京市科学技术研究院“北萌芽”计划项目(11000022T000000455559)
北京市科学技术研究院市级财政项目(11000022T000000442954)。
