摘要
目的:观察夹脊电针对神经根型颈椎病(CSR)模型大鼠的作用效应,从Nrf2/ARE通路探究其作用机制。方法:将75只SPF级SD大鼠随机分为空白对照组、假手术组、模型组、针刺组和电针组。空白对照组不予任何处理,用手术方法将用福尔马林溶液浸泡过的定量滤纸片放在C 8神经根腋下建立CSR型,假手术组仅暴露神经根,不放置滤纸片。造模完成后观察各组大鼠的疼痛行为表现、步态障碍评分和抓握能力评分,从造模后第4天开始,假手术组和模型组予以每日捆绑15 min,针刺组予以每日捆绑+针刺15 min,电针组予以每日捆绑+电针15 min。治疗结束后用试剂盒检测大鼠血清中丙二醛(MDA)、超氧化物歧化酶(SOD)和谷胱甘肽过氧化物酶(GSH-PX)的含量或活力,运用Western blot技术检测大鼠神经根组织中核因子E2相关因子(Nrf2)、抗体Kelch样ECH相关蛋白(Keap1)和血红素氧合酶(HO-1)的表达。结果:针刺组和电针组大鼠疼痛行为表现、步态障碍和抓握能力改善程度和好转速度均优于模型组。与空白对照组比较,假手术组大鼠血清MDA的含量、SOD与GSH-PX的活性差异无统计学意义(P>0.05)。与假手术组比较,模型组大鼠血清MDA的含量显著升高,SOD与GSH-PX的活性显著降低,并且神经根组织Nrf2与HO-1表达显著下降,Keap1表达显著上升,差异具有统计学意义(P<0.01)。与模型组比较,针刺组和电针组大鼠血清MDA的含量明显降低,SOD的活性明显升高,差异具有统计学意义(P<0.05),GSH-PX的活性显著升高,差异具有统计学意义(P<0.01),神经根组织Nrf2与HO-1的表达显著上升,Keap1的表达显著下降,差异具有统计学意义(P<0.01)。与针刺组比较,电针组大鼠血清MDA的含量明显升高,SOD与GSH-PX的活性明显降低,差异具有统计学意义(P<0.05),神经根组织Nrf2的表达显著上升,差异具有统计学意义(P<0.01),HO-1的表达明显上升,差异具有统计学意义(P<0.05),Keap1的表达显著下降,差异具有统计学意义(P<0.01)。结论:电针夹脊穴可以改善CSR大鼠的步态障碍,减轻CSR大鼠的根性疼痛,其机制可能与夹脊电针激活Nrf2/ARE信号通路,上调Nrf2、HO-1的表达和下调Keap1的表达水平有关。
Objective:To observe the effect of Jiaji electro-acupuncture(EA)in treatment of cervical spondylotic radiculopathy(CSR)in rats and to investigate its action mechanism in terms of Nrf2/ARE pathway.Methods:75 SPF-grade SD rats were randomly divided into the blank control group,the sham operation group,the model group,the acupuncture group and the EA group.The blank control group was not given any intervention,and the CSR model was established by surgically placing quantitative filter paper pieces soaked with formalin solution under the axilla of C8 nerve roots;whereas the sham operation group only exposed the nerve roots without placing filter paper pieces.The pain behavior,gait disturbance score and grasping ability score of rats in each group were observed after modeling.From the fourth day after modeling,the sham operation group and the model group were given daily binding for 15 minutes,the acupuncture group was given daily binding plus acupuncture for 15minutes,and the EA group was given daily binding plus EA for 15minutes.After the treatment,the serum content of MDA and the activities of SOD and GSH-PX were detected by kits,and the expressions of Nrf2,Keap1 and HO-1 in rat nerve root tissues were detected by Western blot technique.Results:The improvement degree and speed of pain behavior,gait disturbance and grasping ability in the acupuncture group and the EA group were more significant than those in the model group.Compared with those in the blank control group,there were no statistically significant differences in the serum levels of MDA and the activities of SOD and GSH-PX in the sham operation group(P>0.05).Compared with those in the sham operation group,the serum content of MDA was significantly higher,activities of SOD and GSH-PX were significantly lower,the expressions of Nrf2 and HO-1 were significantly decreased,and the expression of Keap1 was significantly increased in the model group(P<0.01).Compared with those in the model group,the serum content of MDA was decreased significantly(P<0.05),the activities of SOD and GSH-PX were increased significantly(P<0.05,P<0.01),the expressions of Nrf2 and HO-1 were significantly increased,and the expression of Keap1 was significantly decreased in the acupuncture group and the EA group(P<0.01).Compared with those in the acupuncture group,the serum content of MDA was significantly higher,the activities of SOD and GSH-PX were significantly lower(P<0.05),the expressions of Nrf2 and HO-1 were increased significantly(P<0.01,P<0.05),and the expression of Keap1 was decreased significantly in the EA group(P<0.01).Conclusion:Jiaji EA can improve gait impairment and reduce pain in CSR rats,and the mechanism may be related to the activation of Nrf2/ARE signaling pathway,up-regulation of Nrf2 and HO-1 expression and down-regulation of Keap1 expression.
作者
杨旭霞
娄宏君
王文韬
高曦
YANG Xuxia;LOU Hongjun;WANG Wentao;GAO Xi(Heilongjiang University of Chinese Medicine,Harbin 150040,China;The First Affiliated Hospital of Heilongjiang University of Chinese Medicine,Harbin 150040,China;Shandong Wengdeng Orthopedic Hospital,Weihai 264499,China)
出处
《针灸临床杂志》
2024年第2期63-68,共6页
Journal of Clinical Acupuncture and Moxibustion
基金
黑龙江省自然科学基金,编号:LH2021H092
黑龙江中医药大学“双一流”学科优秀青年基金,编号:HLJSYL22011。
关键词
夹脊
电针
神经根型颈椎病
核因子E2相关因子2
抗氧化反应元件
氧化应激
Jiaji
Electro-acupuncture
Cervical spondylotic radiculopathy
Nuclear factor E2-related factor 2
Antioxidant response element
Oxidative stress
