摘要
目的比较急性痛风性关节炎患者与健康对照组外周血脂联素及CD4+T细胞亚群的差异,并探究痛风患者脂联素与血尿酸、疾病活动度、CT4^(+)T细胞亚群及部分细胞因子的相关性。方法收集急性痛风组(n=90)及健康对照组(n=72)的临床资料(包括一般资料、中性粒细胞、红细胞沉降率、C反应蛋白、血尿酸、CT4^(+)T细胞亚群及部分细胞因子);检测两组外周血脂联素水平,比较两组间外周血脂联素及CD4+T细胞亚群的差异;分析脂联素与各临床资料的相关性。结果与健康对照组比较,急性痛风组外周血脂联素水平明显降低(P<0.001),且Th2、Th17、Th17/Treg均升高(P<0.05),Treg、Th1/Th2均降低(P<0.05)。急性痛风组外周血脂联素与中性粒细胞、红细胞沉降率及C反应蛋白均呈负相关(r=-0.244,P<0.05;r=-0.311,P<0.05;r=-0.506,P<0.001),而与血尿酸无相关性。急性痛风组外周血脂联素与Th1和Th1/Th2均呈正相关(r=0.252,P<0.05;r=0.218,P<0.05)。急性痛风组外周血脂联素与白细胞介素-2(interleukin-2,IL-2)、白细胞介素-4(interleukin-4,IL-4)和白细胞介素-10(interleukin-10,IL-10)均呈正相关(r=0.323,P<0.05;r=0.377,P<0.05;r=0.359,P<0.05);而与白细胞介素-6(interleukin-6,IL-6)和肿瘤坏死因子-α(tumor necrosis factor-α,TNF-α)呈负相关(r=-0.265,P<0.05;r=-0.299,P<0.05)。多元线性回归分析显示,急性痛风组外周血脂联素与红细胞沉降率、C反应蛋白、IL-6及TNF-α均呈负相关(B _(ESR)=-12.541,P=0.003;B_( CRP)=-8.256,P=0.024;B_( IL-6)=-15.907,P=0.037;B _(TNF-α)=-79.770,P=0.040),而与Th1呈正相关(B _(Th1)=2.959,P=0.006)。结论急性痛风性关节炎患者外周血脂联素水平降低,Th2和Th17升高,而Treg降低,脂联素降低与急性痛风性关节炎患者免疫紊乱及免疫炎症相关。
Objective To compare the difference of peripheral blood adiponectin and CD4+T cell subsets between patients with acute gouty arthritis and healthy controls,to explore the correlation between adiponectin and serum uric acid,disease activity,CT4^(+)T cell subsets and some cytokines in patients with gout.Methods The clinical data(including general data,neutrophils,erythrocyte sedimentation rate,C-reactive protein,blood uric acid,CT4^(+)T cell subsets and some cytokines)of acute gout group(n=90)and healthy control group(n=72)were collected.The level of adiponectin in peripheral blood of two groups were detected,and the differences of adiponectin and CD4^(+)T cell subsets between the two groups were compared;the correlation between adiponectin and clinical data was analyzed.Results The levels of serum adiponectin in the acute gout group were significantly lower than those in the healthy control group(P<0.001),and the levels of Th2,Th17,Th17/Treg were significantly higher than those in the healthy control group(P<0.05),while the levels of Treg and Th1/Th2 were significantly lower than those in the healthy control group(P<0.05).In the acute gout group,adiponectin was negatively correlated with neutrophil,erythrocyte sedimentation rate and C-reactive proten(r=-0.244,P<0.05;r=-0.311,P<0.05;r=-0.506,P<0.001),there was no correlation with serum uric acid.In acute gout group,adiponectin was positively correlated with Th1 and Th1/Th2(r=0.252,P<0.05;r=0.218,P<0.05).In acute gout group,adiponectin in peripheral blood was positively correlated with interleukin-2(IL-2),interleukin-4(IL-4)and interleukin-10(IL-10)(r=0.323,P<0.05;r=0.377,P<0.05;r=0.359,P<0.05).There was a negative correlation between interleukin-6(IL-6)and tumor necrosis factor-α(TNF-α)(r=-0.265,P<0.05;r=-0.299,P<0.05).Multiple linear regression analysis showed that adiponectin in the acute gout group was negatively correlated with erythrocyte sedimentation rate,C-reactive protein,IL-6 and TNF-α(B _(ESR)=-12.541,P=0.003;B _(CRP)=-8.256,P=0.024;B _(IL-6)=-15.907,P=0.037;B _(TNF-α)=-79.770,P=0.040),but positively correlated with Th1(B Th1=2.959,P=0.006).Conclusion The levels of adiponectin in the peripheral blood of patients with acute gouty arthritis were decreased,the levels of Th2 and Th17 were increased,and the levels of Treg were decreased.The decrease of adiponectin was related to the immunological disorder and inflammation in the patients with acute gouty arthritis.
作者
王颖
高惠英
张琪
尚莉丽
范春雪
罗静
王彩虹
WANG Ying;GAO Huiying;ZHANG Qi(Department of Rheumatology,Second Hospital of Shanxi Medical University,Shanxi 030001,China)
出处
《医学研究杂志》
2024年第2期96-100,共5页
Journal of Medical Research
基金
山西省重点研发计划项目(201803D31130)。
