摘要
以正红菇(Russula Vinosa Linolbl)为原材料,采用超声波辅助法提取正红菇中所含多酚,通过单因素和响应面实验对正红菇多酚的提取工艺进行优化。结果表明,正红菇多酚的最佳提取工艺为:液料比36∶1,乙醇体积分数59%,超声时间62 min,超声温度51℃。通过DPPH自由基清除率的测定,正红菇多酚清除DPPH自由基半抑制率(IC_(50))值为0.2438μg/mL,表明正红菇多酚提取物具有较好的抗氧化活性。
Using Russula vinosa linolbl as raw material,the polyphenols were extracted by ultrasonic-assisted method.The extraction process of polyphenols from Russula vinosa linolbl was optimized by single factor and response surface methodology.The results showed that the optimal extraction process of polyphenols from Russula vinosa linolbl was as follows:liquid-solid ratio was 36∶1,ethanol volume fraction was 59%,ultrasonic time was 62 min,ultrasonic temperature was 51℃.According to the determination of DPPH free radical scavenging rate,the half inhibition rate(IC_(50))of polyphenols from Russula vinosa linolbl was 0.2438μg/mL,which indicated that the polyphenol extract of Russula vinosa linolbl had good antioxidant activity.
作者
李科鹏
冯玉会
普开仙
李锐扬
戴应淑
师伟
李琛
LI Ke-peng;FENG Yu-hui;PU Kai-xian;LI Rui-yang;DAI Ying-shu;SHI Wei;LI Chen(School of Chemistry,Biology and Environment,Yuxi Normal University,Yunnan Yuxi 653100;School of Physics and Electronic Engineering,Yuxi Normal University,Yunnan Yuxi 653100,China)
出处
《广州化工》
CAS
2023年第19期11-15,共5页
GuangZhou Chemical Industry
基金
云南省应用基础研究计划面上项目(202101AT070694)
玉溪师范学院大学生创新创业训练计划项目(2021A019)
云南省高校特色植物化学评价及应用科技创新团队支持计划。
关键词
正红菇
多酚
超声波辅助法
响应面
抗氧化
Russula vinosa linolbl
polyphenols
ultrasonic-assisted method
response surface
antioxidant
