摘要
本试验旨在低鱼粉饲料中添加酶解鱼小肽,研究其对牛蛙蝌蚪生长、变态、肝脏代谢及抗氧化能力的影响。试验挑选4800尾初始体重为0.04 g左右的牛蛙蝌蚪,随机分成5组,每组3个重复,每个重复320尾牛蛙蝌蚪,分别饲喂5种不同的等氮等脂饲料,其中以15%鱼粉饲料(FM15组)为对照,另设置2个不同鱼粉水平饲料,鱼粉水平分别为10%、5%,并在每个鱼粉水平饲料中分别添加2.5%和5.0%酶解鱼小肽,分别记为F10E2.5、F10E5、F5E2.5、F5E5组,试验投喂持续65 d。结果显示:1)与FM15组相比,低鱼粉水平饲料中添加酶解鱼小肽均能提高蝌蚪的变态率和存活率,其中F10E5组牛蛙蝌蚪变态率显著提高(P<0.05)。2)与FM15组相比,除F5E2.5组外其余3组肠道脂肪酶活性均显著提高(P<0.05)。3)与FM15组相比,饲料中添加酶解鱼小肽均能显著提高肠道超氧化物歧化酶(SOD)活性(P<0.05),此外F10E5组肠道谷胱甘肽过氧化物酶(GSH-Px)、过氧化氢酶(CAT)活性及谷胱甘肽(GSH)含量显著提高(P<0.05)。4)与FM15组比,饲料中添加酶解鱼小肽均能显著提升肠道磺基转移酶(SULT)基因相对表达量(P<0.05),此外F10E5组肠道CAT、SOD及谷胱甘肽S-转移酶(GST)基因相对表达量显著提升(P<0.05)。5)除F5E5组外,饲料中添加酶解鱼小肽均能改善肝细胞空泡化。6)与FM15组相比,饲料中添加酶解鱼小肽均能显著提高肝脏谷草转氨酶与酸性磷酸酶活性(P<0.05),此外添加5.0%酶解鱼小肽后肝脏谷丙转氨酶活性均显著提高(P<0.05)。综上所述,饲料中添加10%鱼粉和5.0%酶解鱼小肽(F10E5)能够在不影响生长的情况下,提高牛蛙蝌蚪变态率、存活率及抗氧化能力。
The aim of this study was to investigate the effects of a low fish meal diet supplemented with enzymatic fish peptides on growth,metamorphosis,liver metabolism and antioxidant capacity of bullfrog tadpole.A total of 4800 tadpoles with an initial body weight of 0.04 g were randomly divided into 5 groups with 3 replicates per group and 320 tadpoles per replicate,and fed 5 different isoniazid and isolipid diets,respectively.A 15%fish meal diet(FM15 group)was set as the control,and two diets with different fish meal levels were set as 10%and 5%,respectively.In each diet,2.5%and 5.0%enzymatic fish peptides were added,which were recorded as F10E2.5,F10E5,F5E2.5 and F5E5 groups,respectively.The experimental feeding lasted for 65 days.The results showed as follows:1)compared with FM15 group,the addition of enzymatic fish peptides could increase the metamorphosis and survival rate of tadpoles,among which the metamorphosis of bullfrog tadpoles in the F10E5 group was significantly increased(P<0.05).2)Compared with the FM15 group,the addition of enzymatic fish peptides except for F5E2.5 group increased intestinal lipase activity of bullfrog(P<0.05).3)Compared with FM15 group,the addition of enzymatic fish small peptides could significantly increase the intestinal superoxide dismutase(SOD)activity of bullfrogs(P<0.05),in addition,the intestinal glutathione peroxidase(GSH-Px),catalase(CAT)activities and glutathione(GSH)content in F10E5 group were significantly increased(P<0.05).4)Compared with FM15 group,the addition of enzymatic fish peptides significantly increased the relative expression level of sulfotransferase(SULT)gene in the intestine of bullfrogs(P<0.05),and the F10E5 group significantly increased the relative expression level of CAT,SOD,and glutathione S-transferase(GST)genes(P<0.05).5)Except for the F5E5 group,the addition of enzymatic fish peptides improved the vacuolisation of bullfrog hepatocytes.6)Compared with FM15 group,the addition of enzymatic fish peptides significantly increased the hepatic aspartate aminotransferase and acid phosphatase activities of bullfrogs(P<0.05),and the hepatic alanine aminotransferase activity of bullfrogs were significantly increased after adding 5.0%enzymatic fish peptides(P<0.05).In conclusion,dietary supplementation of 10%fish meal and 5.0%enzymatic fish peptides(F10E5)can improve metamorphosis rate,survival rate and antioxidant capacity of bullfrog tadpoles without affecting growth.
作者
徐文杰
朱波
蔡明浪
戴济鸿
钟蕾
邵闯
胡毅
陈开健
XU Wenjie;ZHU Bo;CAI Minglang;DAI Jihong;ZHONG Lei;SHAO Chuang;HU Yi;CHEN Kaijian(Fisheries College,Hunan Agricultural University,Changsha 410128,China)
出处
《动物营养学报》
CAS
CSCD
北大核心
2024年第1期478-489,共12页
CHINESE JOURNAL OF ANIMAL NUTRITION
基金
湖南省重大专项(2017NK1030)。
关键词
酶解鱼小肽
牛蛙蝌蚪
生长
变态
抗氧化
enzymatic fish peptides
bullfrog tadpoles
growth
metamorphosis
antioxidant
