摘要
目的应用高频超声观察离体猪胃壁分层,分析其与显微外科解剖结构及组织学病理分层的对应关系。方法应用外科手术显微镜、高频超声(探头频率:14 MHz、16 MHz、20 MHz、40 MHz)及组织学病理显微镜观察离体猪胃壁分层;于40 MHz频率超声探头下观察离体猪胃壁第3层强回声中低回声厚度;清洗刮除离体猪胃壁表面黏液,超声观察胃壁第1层强回声变化;使用发丝分层穿入胃壁组织,观察离体猪胃壁超声表现与显微外科解剖结构及组织学病理分层的对应关系。结果外科手术显微镜下显示,离体猪胃壁自上向下大致分为5层,第1层呈浅粉色,第2层呈略深粉色,第3层呈白色,第4层呈肉橘色,第5层呈白色;超声表现为强-低-强-低-强5层回声,于20~40 MHz频率超声探头下观察离体猪胃壁示第3层强回声中可探及一低回声,其中于40 MHz频率超声探头下测量其厚度为(0.611±0.041)mm,与组织学病理测量的离体猪胃壁黏膜肌层厚度[(0.597±0.027)mm]比较差异无统计学意义(t=0.907,P=0.376);清洗刮除离体猪胃壁表面黏液前、后第1层强回声厚度[(0.052±0.008)cm vs.(0.019±0.003)cm]比较差异有统计学意义(t=12.279,P<0.001)。发丝分层穿入离体猪胃壁组织学病理显示,胃壁黏膜固有层、黏膜下层及肌层中各可见一空洞,与穿过发丝后的超声表现及显微外科解剖结构对应发丝的位置相仿。结论高频超声可较好地显示离体猪胃壁的组织分层,并与显微外科解剖结构及组织学病理分层对应,可为临床诊断胃壁各层病变提供解剖学依据。
Objective To observe the layers of pigs isolated porcine gastric wall by high-frequency ultrasound,and to analyze the relationship between ultrasonic observation and microsurgical anatomy and histological pathology.Methods The layers of pigs isolated porcine gastric wall were observed by the surgical microscope,high-frequency ultrasound(at frequencies of 14 MHz,16 MHz,20 MHz and 40 MHz)and histological microscope.The ultrasound probe at the frequency of 40 MHz was used to observe the thickness of low echo in hyperechogenicity of the third layer of isolated porcine gastric wall.The changes of the first layer of the gastric wall before and after the removal of mucus were observed by ultrasonography.The hair was utilized for transgressing the gastric wall,and the correlation between the ultrasonic manifestations of the isolated porcine gastric wall and the stratification observed through microsurgical anatomy and histological pathology.Results The layers of pigs isolated porcine gastric wall observed by the surgical microscope,consisted of approximately 5 layers from top to botton.The first layer appeared as light pink,followed by slightly darker pink in the second layer.The third layer appeared as white,while the fourth layer appeared as orange tint resembling meat,the fifth layer appeared as white.The high-frequency ultrasound showed a distinct pattern of 5 echo layers:strong,low,strong,low and strong.Within the third layer of strong echo observed by the 20~40 MHz ultrasonic probe,the thickness of this low echo was(0.611±0.041)mm by 40 MHz ultrasonic probe,and the thickness of muscularis mucosae measured by pathology was(0.597±0.027)mm,there was no significant difference(t=0.907,P=0.376).The thickness of the first layer of the gastric wall exhibited a significant difference before and after the removal of the mucus[(0.052±0.008)cm vs.(0.019±0.003)cm,t=12.279,P<0.001].The histological pathology of the porcine stomach showed that a hole in the lamina propria,the submucosa and the muscle layer of the gastric wall,which was similar to the position of the ultrasound findings and the microsurgical anatomy after crossing the hair.Conclusion The utilization of high-frequency ultrasound enables the visualization of distinct tissue layers within the pigs gastric wall,thereby establishing a solid anatomical foundation for the accurate diagnosis of gastric wall lesions.
作者
薛静静
王泽城
陈永健
李少辉
吕国荣
XUE Jingjing;WANG Zecheng;CHEN Yongjian;LI Shaohui;LYU Guorong(Department of Ultrasound,the Second Affiliated Hospital of Fujian Medical University,Fujian 362000,China)
出处
《临床超声医学杂志》
CSCD
2024年第2期95-99,共5页
Journal of Clinical Ultrasound in Medicine
关键词
超声检查
组织学病理
分层
显微外科
离体胃壁
猪
Ultrasonography
Histological pathology
Stratification
Microsurgery
Isolated porcine gastric wall,pigs
