摘要
【目的】肠道氧化损伤对机体健康和生长发育具有严重危害。本研究旨在比较45 d肉鸡不同肠段H_(2)O_(2)含量,并通过盲肠急性灌注过氧化氢酶(CAT)研究其对雏鸡肠道健康与氨氮代谢的影响,以期为肠道靶向氧化应激的营养调控提供新思路。【方法】试验选用21 d爱拔益加(AA)鸡30羽,不分组,饲喂至45 d后屠宰并采集各肠段内容物测定H_(2)O_(2)含量。另选用1 d快大三黄肉鸡饲喂至1 w后,根据体质量相近原则抽取24羽,随机分为对照组、低剂量组(1 U/g组)和高剂量组(10 U/g组),每组8个重复,每重复数1羽。根据体质量大小,注射质量分数20%麻醉剂,剂量为10µL/g;麻醉后,对照组两侧盲肠灌注2µL/g生理盐水;低剂量组左侧盲肠灌注1 U/g CAT(处理侧,Treat),右侧盲肠灌注等体积生理盐水(对照侧,Paired control);高剂量组左侧盲肠灌注10 U/g CAT,右侧盲肠灌注等体积生理盐水。灌注3 h后,采集颈动脉、门静脉、两侧盲肠内容物及黏膜用于测定肠道氧化还原、氨氮代谢、炎症与屏障以及肠道健康相关指标,采集盲肠切片样用于苏木素-伊红法(H&E)染色。【结果】45 d AA肉鸡回肠、盲肠和结肠中的H_(2)O_(2)含量显著高于十二指肠和空肠。在盲肠灌注雏鸡试验中,与对照侧盲肠相比,低剂量组处理侧提高了内容物H_(2)O_(2)含量,高剂量组处理侧具有降低内容物H_(2)O_(2)、肠黏膜和血清MDA的趋势。氨氮代谢结果表明,盲肠灌注CAT能降低血氨水平,增加尿素水平,且高剂量组处理侧能显著降低内容物氨含量、提高尿素水平。液相色谱法检测肠道内容物有机酸结果发现,高剂量组处理侧具有降低内容物苹果酸含量的趋势,但对乙酸和琥珀酸含量无明显影响。【结论】45 d AA肉鸡回肠、盲肠和结肠中的H_(2)O_(2)含量显著高于十二指肠和空肠。雏鸡盲肠灌注10 U/g CAT可能通过降低内容物H_(2)O_(2)、肠黏膜和血清MDA,改善肠道氧化还原状态,同时促进氨向尿素转化。
[Objective]Intestinal oxidative damage is of significant harm to body health and growth,and the degree of intestinal oxidative damage varies among different intestinal segments.Excessive H_(2)O_(2) produced by microbial metabolism leads to increased intestinal oxidative stress.This study aims to compare the H_(2)O_(2) content in different intestinal segments of broilers and study its effects on intestinal health and ammonia nitrogen metabolism of chicks by acute caecal perfusion,thus providing a new idea for nutritional regulation targeting intestinal oxidative stress.[Method]3021-day-old AA chickens were selected and slaughtered on the 45th day of the experiment,and the contents of each intestinal segment were collected to determine the content of H_(2)O_(2).In addition,1-day fast-growing Sanhuang chicken broilers were selected for feeding until one week and randomly divided into control group,low-dose group and high-dose group with 8 replicates per group and the number of replicates per group was 1.After anesthesia,normal saline of 2µL/g was injected into both sides of the caecum in the control group,1 U/g CAT was injected into the left caecum in the low-dose group,10 U/g CAT was injected into the left caecum in the high-dose group,and the normal saline was injected into the right caecum in both groups with the equal volume.3 h after perfusion,carotid artery,portal vein,bilateral cecal contents and cecal mucosa were collected to determine intestinal REDOX,ammonia nitrogen metabolism,inflammation and barrier,and intestinal health related indicators.Cecum sections were collected for hematoxylin-eosin(H&E)staining.[Result]The results showed that H_(2)O_(2) content in ileum,cecum and colon of broilers was significantly higher than that in duodenum and jejunum.Compared with the control side cecum,the infusion of low-dose CAT increased the content of H_(2)O_(2),while the infusion of high-dose CAT had a tendency to decrease the content of H_(2)O_(2),intestinal mucosa and serum MDA.The results of ammonia nitrogen metabolism showed that caecal infusion of CAT could decrease blood ammonia level and increase urea level.High dose of CAT can significantly reduce ammonia content and increase urea level.The results of liquid chromatography showed that the high dose CAT treatment group had a tendency to reduce the content of malic acid,but it had no significant effect on the content of acetic acid and succinic acid.[Conclusion]The H_(2)O_(2) content in ileum,cecum and colon of broilers was significantly higher than that in duodenum and jejunum.Infusion of 10 U/g CAT into chicken cecum may improve intestinal REDOX status by reducing content H_(2)O_(2),intestinal mucosa and serum MDA,and promote the conversion of ammonia to urea.
作者
江宏凤
杨雪
朱云龙
韩国清
江青艳
束刚
JIANG Hongfeng;YANG Xue;ZHU Yunlong;HAN Guoqing;JIANG Qingyan;SHU Gang(Guangdong Laboratory of Lingnan Modern Agriculture and Guangdong Province Key Laboratory ofAnimal Nutritional Regulation,College of Animal Science,South China Agricultural University,Guangzhou510642,China;Foshan Huayang Animal Nutrition Co.,Ltd.,of Guangdong Province Foshan,Guangdong528100,China)
出处
《江西农业大学学报》
CAS
CSCD
北大核心
2023年第6期1463-1472,共10页
Acta Agriculturae Universitatis Jiangxiensis
基金
国家“十四五”重点研发项目(2022YFD1300401)。
关键词
肉鸡
肠道健康
氨氮代谢
过氧化氢酶
盲肠
broiler
intestinal health
ammonia nitrogen metabolism
catalase
cecum
