摘要
目的:综合评价金花茶花的降血糖和抗氧化作用。方法:制备金花茶花水提物(aqueous extract of Camellia nitidissima flower,CFA),利用高效液相色谱-质谱联用法进行组分鉴定;同时,建立2型糖尿病小鼠模型并随机分为模型组,阿卡波糖阳性组(20 mg/kg mb),CFA低、中、高剂量组(200、400、800 mg/kg mb)。灌胃干预5周后,分析各组小鼠一般生长特征,血清中血糖、空腹胰岛素及血脂水平,胰、肝脏氧化应激水平、组织形态变化及细胞凋亡情况。结果:金花茶花具有较高的多酚、多糖含量,清除1,1-二苯基-2-三硝基苯肼自由基和抑制α-葡萄糖苷酶的半抑制浓度分别为(24.14±0.64)、(69.99±1.97)μg/m L,从金花茶花中鉴定出7种主要化合物。此外,金花茶花可有效改善糖尿病小鼠“三多一少”症状,显著降低小鼠空腹血糖、餐后血糖、总胆固醇、甘油三酯、低密度脂蛋白胆固醇、丙二醛水平,升高胰岛素、高密度脂蛋白胆固醇的含量,提升总超氧化物歧化酶、谷胱甘肽过氧化物酶的活性,减轻胰脏、肝脏组织的病理损伤。结论:金花茶花对2型糖尿病小鼠具有显著的降血糖及抗氧化作用。
Objective:To comprehensively evaluate the hypoglycemic and antioxidant effects of Camellia nitidissima flower.Methods:The chemical constituents in the aqueous extract of C.nitidissima flower(CFA)were identified by high performance liquid chromatography-mass spectrometry(HPLC-MS).Next,a mouse model of type 2 diabetes was established,and the diabetic mice were randomly divided into five groups:model,positive control(acarbose at 20 mg/kg mb),low-,medium-and high-dose CFA(200,400 and 800 mg/kg mb,respectively).After five weeks of intragastric intervention,general growth characteristics,serum glucose,fasting insulin(FINS)and lipid levels,oxidative stress in pancreas and liver tissues,tissue morphological changes and cell apoptosis were analyzed.Results:CFA had a high content of polyphenols and polysaccharides.The half-maximal inhibitory concentration(IC50)values for scavenging of 1,1-diphenyl-2-picrylhydrazyl(DPPH)radical andα-glucosidase inhibition were(24.14±0.64)and(69.99±1.97)μg/mL,respectively.Seven compounds were identified from CFA.In addition,CFA could effectively improve the‘three more and one less’symptoms of diabetic mice,significantly reduce the levels of fasting blood glucose(FBG),postprandial blood glucose(PBG),total cholesterol(TC),triglyceride(TG),low-density lipoprotein cholesterol(LDL-C)and malondialdehyde(MDA),increase the levels of insulin and high-density lipoprotein cholesterol(HDL-C),improve the activities of total superoxide dismutase(SOD)and glutathione peroxidase(GSH-Px),and alleviate pathological damage in pancreas and liver tissues.Conclusion:CFA has significant hypoglycemic and antioxidant effects on type 2 diabetic mice.
作者
刘芬芬
蒲首丞
赵雯靓
王怡婷
薛琛
徐丽珊
LIU Fenfen;PU Shoucheng;ZHAO Wenliang;WANG Yiting;XUE Chen;XU Lishan(College of Life Sciences,Zhejiang Normal University,Jinhua 321004,China;Zhejiang Provincial Key Laboratory of Biotechnology on Specialty Economic Plants,Jinhua 321004,China)
出处
《食品科学》
EI
CAS
CSCD
北大核心
2024年第3期94-101,共8页
Food Science
基金
金华市科技计划项目(2020-2-014)。
关键词
金花茶花
2型糖尿病
糖脂代谢
氧化应激
Camellia nitidissima flower
type 2 diabetes
glucose and lipid metabolism
oxidative stress
