摘要
为了建立气相色谱法(GC)测定国公酒中7种掺伪香精成分的分析方法,对提取溶剂、提取溶剂体积、氯化钠固体加入量以及色谱柱等参数进行考察,以氢火焰离子化检测器(FID)进行检测,外标法定量。结果表明,最优测定条件为采用10 mL乙酸乙酯作为提取溶剂,氯化钠添加量为3 g,InertCap 624毛细管色谱柱(30 m×0.25 mm×0.25μm)分离,分流比5∶1,在此条件下7种香精成分能实现完全分离,线性关系良好,相关系数R2均大于0.999。在1.0~10 mg/L范围内,7种香精成分的加标回收率为82.0%~98.1%,精密度实验结果的相对标准偏差(RSD)均低于10%,精密度及准确度良好。该方法简单、快速、准确,可满足国公酒中7种香精成分的检测。
In order to establish a gas chromatography(GC)method for the determination of 7 kinds of adulterated flavor components in Guogong liquor,the extraction solvent,extraction solvent volume,sodium chloride solid addition and chromatographic column were investigated.The parameters were detected by hydrogen flame ionization detector(FID)and quantified by external standard.Results showed that the optimal determination conditions were using 10 ml ethyl acetate as the extraction solvent,adding 3 g sodium chloride,separating with InertCap 624 capillary column(30 m×0.25 mm×0.25μm)with a split ratio of 5∶1,the 7 essence components could be completely separated,with good linear relationship,and R2 were all greater than 0.999.In the range of 1.0-10 mg/L,the adding standard recovery was between 82.0%and 98.1%,and the relative standard deviation(RSD)of precision tests results were all below 10%.The method was simple,rapid and accurate,and could be used to detect 7 essence ingredients in Guogong liquor.
作者
郑利军
邓晓庆
陈雄
方宣启
唐万里
唐文杰
陈同强
ZHENG Lijun;DENG Xiaoqing;CHEN Xiong;FANG Xuanqi;TANG Wanli;TANG Wenjie;CHEN Tongqiang(Zhongda Intelligent Technology Co.,Ltd.,Changsha 410000,China;Hunan Zhenhua Inspection Technology Co.,Ltd.,Changsha 410000,China;Hunan Province Produce Commodity Quality Testing&Research Institute,Changsha 410007,China)
出处
《中国酿造》
CAS
北大核心
2024年第2期253-257,共5页
China Brewing
基金
湖南省自然科学基金项目(2022JJ90024)
食品安全快速检测与溯源关键技术研究与示范(2020SK2128)。
关键词
气相色谱
国公酒
掺伪
香精
测定
gas chromatography
Guogong liquor
adulteration
essence
determination
