摘要
建立HPLC法同时测定新乐康片中三味原料药材钩藤、酸枣仁和萝芙木中钩藤碱、异钩藤碱、斯皮诺素、酸枣仁皂苷A、酸枣仁皂苷B、育亨宾、阿义吗啉及利血平等8种活性成分的含量。采用Titank C18(250 mm×4.6mm,5μm)色谱柱,以乙腈-0.4%磷酸水溶液(含0.05%三乙胺)为流动相,梯度洗脱,检测波长204 nm,流速为1.0 mL/min,柱温为30℃。上述三味药材相关成分之间分离度良好,在λ=204 nm条件下,其在分别的线性范围内与响应值之间的线性关系良好(R^(2)≥0.9990);8种活性成分的平均加样回收率98.37%~101.21%,RSD<2%,仪器精密度、供试品溶液的稳定性与方法重复性均良好。建立的HPLC法可一法对钩藤、酸枣仁和萝芙木三味中药材中8种活性成分含量进行测定,为药业在生产新乐康片前期选材上节约了测定时间,提高了生产效率,同时为新乐康片中活性成分HPLC含量测定方法的建立及其质量标准升级提供参考。
To establish HPLC method to determine the content of eight active ingredients,namely,alkali,sponine,sinin,sinin A,B,Yohimbin,morpholine and reserpine in Xinlekang tablets.Using Titank C18(250 mm×4.6 mm,5μm)column,acetonitrile-0.4%aqueous phosphoric acid solution(containing 0.05%triethylamine)as mobile phase,gradient elution,detection wavelength of 204 nm,the flow rate is 1.0 mL/min,and the column temperature is 30℃.The separation between the related components of the three medicinal materials was good,in the linear range(R^(2)≥0.9990);the average sample recovery of the eight active components was 98.37%~101.21%,RSD<2%,the precision of the instrument and the stability of the test solution and the method were good.The HPLC method can measure the content of 8 active ingredients in uncintus,jujube kernel and lufumu,for the pharmaceutical industry saves the determination time,improves the production efficiency,and provides the determination method of HPLC content and its quality standard upgrade.
作者
邓雨荷
张庆林
杨明武
陈伟
何珺
DENG Yuhe;ZHANG Qinglin;YANG Mingwu;CHEN Wei;HE Jun(School of Pharmacy,Guizhou University,Guiyang 550025,China;Engineering Research Center for the Development and Utilization of Southwest Characteristic Pharmaceutical Biological Resources Ministry of Education,Guiyang 550025,China;Shanxi Taiyuan Pharmaceutical Co.LTD.,Taiyuan 030021,China)
出处
《广州化学》
CAS
2024年第1期75-78,I0004,共5页
Guangzhou Chemistry
关键词
钩藤
酸枣仁
萝芙木
钩藤碱
酸枣仁皂苷A
利血平
高效液相色谱
Uncaria rhynchophylla
Ziziphi Spinosae Semen
Rauvolfia
Rhynchophylline
Jujuboside A
Reserpine
high performance liquid chromatography
