摘要
目的 分析泉州市某区集贸摊点和超市食品中致泻性大肠埃希菌携带情况。方法 选择2021年1—12月从泉州市鲤城区超市以及集贸摊点采集的100份食品样本作为研究对象,严格按照GB 4789—2016《食品安全国家标准食品微生物检验致泻性大肠埃希菌检验》对所有标本开展致泻性大肠埃希菌微生物检验以及毒力基因检测,统计最终的检测结果。比较不同食品类型的致泻性大肠埃希菌检出率差异。结果 100份检测样本当中,总共20份检出致泻性大肠埃希菌,检出率为20.00%;其中包含产肠毒性大肠埃希菌(entero toxigenic Escherichia coli,ETEC)共6株,占比为30.00%;肠致病性大肠埃希菌(entero pathogenic Escherichia coli,EPEC)共5株,占比为25.00%;肠出血性大肠埃希菌(entero hemorrhagic Escherichia coli,EHEC)共5株,占比为25.00%;肠侵袭性大肠埃希菌(entero invasive Escherichia coli,EIEC)共4株,占比为20.00%。牲畜肉类、生禽肉类、凉拌类、海产类以及蔬菜类食品的致泻性大肠埃希菌检出率依次为38.89%、45.00%、5.00%、10.00%、4.55%,其中牲畜肉类、生禽肉类的致泻性大肠埃希菌检出率高于其他食品类型(P <0.05)。分离获得的致泻性大肠埃希菌20株内,出现EHEC的O157共5株;血清凝集予以H7鉴定,其中共有5株EHEC的O157:H7鉴定血清凝集,1株O157在羊肉上分离获得,SLT2、hly、eaeA毒力基因均在H7菌中携带,其他O157共4株,4种毒力基因均为阴性。结论 严格按照有关标准开展微生物检验能有效检出食品中的大肠埃希菌以及致泻性大肠埃希菌毒力基因,有关部门应该要加强卫生监督的力度,确保人民群众的食品安全。
Objective To study the diarrheagenic Escherichia coli detection conditions in the fair trade market stalls and the supermarkets of a district of Quanzhou city.Methods A total of 100 food samples collected from supermarkets and market stalls in Licheng district,Quanzhou city from January to December 2021 were selected as the research objects.The microorganism examination was performed according to National Criteria for Food Safety,Food Microbe Testing of Diarrheagenic Escherichia coli(GB 4789—2016).Diarrheagenic Escherichia coli microorganism and virulence gene examinations were performed.According to the final examination results,Escherichia coli detection rates in the different food samples were recorded.Results A total of 20food samples were discovered with Escherichia coli(20.00%).There were 6 strains of entero toxigenic Escherichia coli(ETEC),accounting for 30.00%.There were 5 strains of entero pathogenic Escherichia coli(EPEC),accounting for 25.00%.There were 5strains of entero hemorrhagic Escherichia coli(EHEC),accounting for 25.00%.There were 4 strains of entero invasive Escherichia coli(EIEC),accounting for 20.00%.Escherichia coli detection rates from the livestock meat,poultry meat,cold dish,marine product and vegetables were 38.89%,45.00%,5.00%,10.00% and 4.55%,Escherichia coli detection rates from the livestock meat and poultry meat were higher than the other food types(P < 0.05).Among 20 isolated Escherichia coli strains,5 EHEC strains were found with O157:H7 serum agglutination,one O157:H7 strain from the mutton was carried with eaeA,hly and SLT2 virulence genes,the resting 4 O157:H7 strains were found with negative eaeA,hly,SLT and SLT2 virulence genes.Conclusion Escherichia coli and its virulence genes can be effectively discovered by the microorganism examination.Therefore,the relevant government departments shall strengthen the health supervision to ensure the people's food safety.
作者
王萍霞
钟煌培
周芳芳
WANG Pingxia;ZHONG Huangpei;ZHOU Fangfang(Laboratory Unit,Quanzhou City Licheng District Center for Disease Control and Prevention,Quanzhou Fujian 362000,China)
出处
《中国卫生标准管理》
2024年第4期24-27,共4页
China Health Standard Management
关键词
大肠埃希菌
致泻性大肠埃希菌
微生物检验
产肠毒性大肠埃希菌
肠致病性大肠埃希菌
肠出血性大肠埃希菌
肠侵袭性大肠埃希菌
Escherichia coli
diarrheagenic Escherichia coli
microorganism examination
entero toxigenic Escherichia coli
entero pathogenic Escherichia coli
entero hemorrhagic Escherichia coli
entero invasive Escherichia coli
