摘要
目的分析不同病原所致肺部感染患儿肺部微生物菌群的差异,探讨肺部微生物菌群与不同病原感染的相关性。方法收集2020年6月至2022年5月因肺部感染行支气管镜诊疗患儿的肺泡灌洗液,利用宏基因组二代测序(mNGS)进行微生物菌群检测,根据最终临床确诊的病原分为肺炎支原体肺部感染(MPP)组、肺炎链球菌组和真菌组。分析比较3组患儿NGS检测结果,从门、属、菌群多样性水平探讨肺部微生物菌群的异同。结果纳入肺炎支原体组25例,肺炎链球菌组13例和真菌组16例。3组患儿的年龄与性别无明显差异(P>0.05)。3组样本在门水平的优势菌群主要有厚壁菌、拟杆菌、变形菌和放线菌,肺炎支原体组拟杆菌门所占比例高于肺炎链球菌组和真菌组,差异有统计学意义(P<0.001);肺炎链球菌组厚壁菌门所占比例明显高于肺炎支原体组和真菌组,差异有统计学意义(P<0.001);真菌组变形菌门和放线菌门所占比例明显高于肺炎支原体组和肺炎链球菌组,差异有统计学意义(P<0.001);肺炎支原体组与肺炎链球菌组的优势菌属为普雷沃菌属、链球菌属、韦荣球菌属,而真菌组为链球菌属、罗氏菌属、颗粒链菌属;肺炎链球菌组α多样性高于真菌组,差异有统计学意义(P<0.05);肺炎链球菌组的β多样性与肺炎支原体组、真菌组有差异,且有一定的临界意义(属水平上:P=0.07;种水平上:P=0.14)。结论不同病原所致肺部感染患儿肺部微生物菌群存在明显差异,拟杆菌门可能有利于肺炎支原体的生长,肺炎链球菌可能抑制其他门类细菌的生长。
Objective To analyze the differences in pulmonary microbiota in children with pulmonary infections of different pathogens,and to explore the correlation between pulmonary microbiota and different pathogen infections.Methods BronchoalveolaRalveolaRlavage fluid(BALF)from children undergoing bronchoscopy for the diagnosis and treatment of pulmonary infections from June 2020 to May 2022 was collected for microbial community detection using the metagenomic next-generation sequencing(mNGS).According to the clinically confirmed pathogen,they were divided into the Mycoplasma pneumoniae pulmonary infection group(MPP group),Streptococcus pneumoniae pulmonary infection group(SPP group)and fungal pulmonary infection group(FP group).NGS detection results of three groups were analyzed.The similarities and differences in pulmonary microbiota at the phylum,genus,and diversity levels were compared.Results There were 25,13 and 16 cases in the MPP group,SPP group and FP group,respectively.No significant differences in age and gendeRwere detected between groups(P>0.05).The dominant microbial communities at the phylum level of the three groups mainly included four types of the Firmicutes,Bacteroides,Proteobacteria and Actinobacteria.The proportion of Bacteroides in MPP group was significantly higher than that of SPP group and FP group(P<0.001).The proportion of Firmicutes in SPP group was significantly higher than that of MPP group and FP group(P<0.001).The proportions of Proteobacteria and Actinobacteria in FP group were significantly higher than those of MPP group and SPP group(P<0.001).The dominant genera of MPP group and SPP group were the Prevotella,Streptococcus and Veillonella,while the dominant genera of FP group were the Streptococcus,Rochella and Streptococcus granulosus.Theαdiversity of SPP group was significantly higher than that the FP group(P<0.05).Theβdiversity of SPP group at the genus(P=0.07)and species level(P=0.14)was different from that of MPP and FP groups,with a certain critical significance.Conclusion There are significant differences in the pulmonary microbial flora of children with pulmonary infections of different pathogens.Bacteroides may be conducive to the growth of Mycoplasma pneumoniae,Streptococcus may inhibit the growth of other bacteria,and Proteobacteria and Actinobacteria may be conducive to the growth of fungi.The role of pulmonary microbial needs to be required further studies for treating pulmonary infectious diseases。
作者
庞明慧
路素坤
黄坤玲
帅金凤
牛波
曹丽洁
褚亚娟
李甜
刘建华
PANG Minghui;LU Sukun;HUANG Kunling(Second Department of Respiratory Medicine,Hebei Children’s Hospital,Affiliated to Hebei Medical University,Hebei,Shijiazhuang 050031,China)
出处
《河北医药》
CAS
2024年第4期520-524,共5页
Hebei Medical Journal
基金
民生科技专项(编号:19277712D)
河北省医学科学研究项目(编号:20210281)。
关键词
肺部微生物菌群
肺部感染
肺炎链球菌
肺炎支原体
真菌
pulmonary microbiota
pulmonary infection
streptococcus pneumoniae
mycoplasma pneumoniae
fungal
