摘要
甜瓜叶片是进行光合作用的主要器官,其形态对甜瓜果实可溶性固形物(糖度)含量高低产生影响显示为了遗传分析与定位甜瓜皱叶基因wl和叶形性状与果实糖度之间的关系,本研究以甜瓜‘西州蜜25号’(X25)(皱叶)为母本,‘伽师’(平叶)为父本构建F_(2)和F_(2)BC_(1)群体,通过χ^(2)检验法对两群体皱、平叶型植株的分离比分析,研究皱叶基因的遗传规律。结果显示,皱叶亲本‘西州蜜25号’(X25)和平叶亲本‘伽师’杂交获得F1代为平叶,说明皱叶对平叶是隐性遗传。利用SSR(Simple sequence repeat)分子标记结合BSA(Bulk segregant analysis)法,构建皱、平叶DNA池,利用全基因组范围内396个SSR分子标记进行多态性标记的筛选,发现6个具有多态性的SSR标记分别位于LG7(CMGA165和GCM344)、LG8(ECM200和CMACC146)和LG10(CMCT134B和CMN22-15)。对F_(2)、F_(2)BC_(1)群体个体基因型检测对甜瓜皱叶基因wl进行连锁遗传分析与定位,最终在LG10上发现一个皱叶基因wl的主效QTL位点(LOD>3),该基因位于标记CMCT134B与SSR2441之间,物理距离为4.4 Mb。将田间获取的皱叶性状与可溶性固形物(糖度)的数据进行分析,糖度测定描述性结果显示,皱叶性状的糖度明显低于平叶性状的糖度,两种表型的糖度存在显著性差异。本研究为甜瓜皱叶性状的精细定位克隆提供参考。
Melon leaf is the main organ of photosynthesis,and its morphology has an effect on the content of soluble solids(sugar content)in melon fruit.In order to genetic analysis and mapping wrinkle leaf gene wl in melon and the relationship between the leaf shape traits and fruit sugar,'Xizhou honey25'(X25)(wrinkled leaf)was the female parent and'Jiashi'(flat leaf)was the male parent to establish F_(2)and F_(2)BC_(1)populations in this study.The separation ratio of wrinkled and flat plants in the two populations was measured byχ^(2)test to analyze the inheritance of wrinkled leaf genes.The results showed that the wrinkled leaf parent'Xizhou honey25'(X25)crossed with the flat leaf parent'Jiashi'(JS)to obtain the F1 generation of flat leaves,which indicate that wrinkled leaf to flat leaf was a recessive inheritance.Simple sequence repeat(SSR)molecular markers combined with bulk segregant analysis(BSA)method were used to construct DNA pools of wrinkles and flat leaves,and 396 SSR molecular markers across the whole genome were used to screen polymorphism markers.Six polymorphic SSR markers were found at LG7(CMGA165 and GCM344),LG8(ECM200 and CMACC146)and LG10(ECM232 and CMCT134B),respectively.The F_(2)and F_(2)BC_(1)genotypes were detected and the wrinkle leaf gene wl of melon was linked analysis and mapped.Finally,a major QTL locus of wrinkle leaf gene wl,which was located between marker CMCT134B and SSR2441(LOD>3),physical distance is 4.4 Mb.The data obtained in the field were analyzed for wrinkled leaf character and soluble solid(sugar content)data.Descriptive results of sugar content determination showed that the sugar content of wrinkled leaf character was significantly lower than that of flat leaf character,and there were significant differences between the two phenotypes.This study provides a reference for fine mapping and cloning of wrinkled leaf traits in melon.
作者
苏比娜·肖克来提
李群
鲁思梦
宁雪飞
周继阳
阮悦
康彩彦
王贤磊
Subina·Xiaokelaiti;Li Qun;Lu Simeng;NingXuefei;Zhou Jiyang;Ruan Yue;Kang Caiyan;Wang Xianlei(College of Life Science and Technology,Xinjiang University,Urumqi,830046)
出处
《分子植物育种》
CAS
北大核心
2024年第5期1480-1487,共8页
Molecular Plant Breeding
基金
国家自然科学基金项目(31660297)
新疆维吾尔自治区高校科研计划项目(XJEDU2021Y010)
自治区天池博士计划科研启动项目(TCBS202034)共同资助。
关键词
甜瓜
皱叶
基因定位
简单序列重复
Melon
Wrinkle leaf
Gene mapping
Simple sequence repeats
