摘要
目的探讨中医滋阴潜阳法对多囊卵巢综合征(polycystic ovarian syndrome,PCOS)伴胰岛素抵抗(insulin resistance,IR)大鼠的磷脂酰肌醇3激酶/蛋白激酶B(phosphoinositide 3-kinase/protein kinase B,PI3K/Akt)信号通路、性激素及胰岛素相关指标的影响。方法以来曲唑加高脂饲料诱导建立多囊卵巢及胰岛素抵抗双重大鼠模型。采用滋阴潜阳法配合达英-35及二甲双胍干预,用免疫荧光法测定卵巢PI3K、Akt通路信号,实时荧光定量PCR检测卵巢PI3K、Akt途径信号分子mRNA表达及PI3K、Akt、胰岛素受体底物-1(insulin receptor substrate 1,IRS-1)、胰岛素受体(insulin receptor,INSR)蛋白表达,性激素、胰岛素生长因子-1(insulin-like growth factor 1,IGF-1),胰岛素敏感指数(insulin sensitivity index,ISI),观察卵巢组织形态变化。结果中医滋阴潜阳法能显著增加卵巢PI3K、Akt mRNA相对表达量(P<0.05),并能显著增加卵巢PI3K、Akt蛋白表达,上调IRS-1、INSR,降低血清睾酮(testos teron,T)、促黄体生成素(luteinizing hormone,LH)含量,调节ISI,增加卵巢颗粒细胞层数及黄体组织数量。结论中医滋阴潜阳法可提高PI3K/Akt通路信号表达水平,改善胰岛素抵抗及卵泡颗粒细胞功能,调节促黄体生成素/卵泡刺激素(LH/FSH)比值,控制LH异常增高,调节多囊卵巢综合征及胰岛素抵抗。
Objective To analyze the effect of traditional Chinese medicine nourishing Yin and subduing Yang method on the phosphoinositide 3-kinase/protein kinase B(PI3K/Akt)signaling pathway,sex hormone and insulin related index in rats with polycystic ovary syndrome(PCOS)and insulin resistance(IR).Method A dual rat model of PCOS and IR was established by inducing with trazole and high-fat diet.Using the method of nourishing Yin and subduing Yang combined with intervention with Diane-35 and metformin,ovarian PI3K and Akt pathway signals were measured by using immunofluorescence,and real-time fluorescence quantitative PCR was used to detect ovarian PI3K and Akt pathway signal molecule mRNA expression,the protein expressions of PI3K,Akt and insulin receptor substrate 1(IRS-1),the levels of insulin receptor(INSR),sex hormones,insulin like growth factor-1(IGF-1)and insulin sensitivity index(ISI)and the changes in ovarian tissue morphology were observed.Result The method of nourishing Yin and subduing Yang could significantly increase the relative expressions of ovarian PI3K and Akt mRNA(P<0.05),and could significantly increase the expressions of ovarian PI3K and Akt protein,up-regulate the levels of IRS-1 and INSR,reduced the levels of serum testosteron(T)and luteinizing hormone(LH)and regulate the level of ISI,and increase the number of granulosa cell layer and corpus luteum.Conclusion The method of nourishing Yin and subduing Yang can increase the signal expression levels of PI3K/Akt pathway,improve IR and follicular granulosa cell function,regulate the ratio of LH/follicle-stimulating hormone(FSH)and control the abnormal increase of LH,and regulate PCOS and IR.
作者
陈旭
王昕
CHEN Xu;WANG Xin(The Affiliated Hospital of Liaoning University of Traditional Chinese Medicine,Shenyang 110032,Liaoning,China)
出处
《中华中医药学刊》
CAS
北大核心
2024年第2期23-28,I0013,共7页
Chinese Archives of Traditional Chinese Medicine
基金
国家自然科学基金项目(82004401)
辽宁省自然科学基金面上项目(2023-MS-226)
辽宁省教育厅面上项目(LJKZ0895)。
关键词
滋阴潜阳法
阴未盛-阳先至
多囊卵巢综合征伴胰岛素抵抗
PI3K/AKT信号通路
nourishing Yin and subduing Yang
Yin being not yet excess-Yang arriving first
polycystic ovary syndrome with insulin resistance
PI3K/Akt signaling pathway
