摘要
研究旨在探究沙棘副产品的营养价值及其提取物对大肠杆菌的抑菌效果。试验采用亚硝酸钠-硝酸铝法测定黄酮含量,醇提法和水提法提取沙棘副产品活性物质,使用滤纸片法、打孔法、肉汤稀释法研究沙棘副产品提取物对大肠杆菌的抑菌效果。结果表明,沙棘副产品干物质(DM)含量为97.98%,灰分(Ash)含量为3.41%,粗蛋白(CP)含量为12.87%,粗脂肪(EE)含量为22.48%。随着沙棘副产品添加量的增加,产气量呈现递减的趋势。醇提法和水提法中常量肉汤稀释法抑菌效果最好;在醇提法中滤纸片法的抑菌效果良好,其抑菌圈为14.6 mm;水提法中滤纸片法无明显抑菌圈;打孔法在醇提法与水提法中均不明显。研究表明,沙棘副产品营养价值丰富,醇提法提取的沙棘副产品活性提取物比水提法对大肠杆菌具有更明显的抑制效果。
The study aimed to explore the nutritional value of sea buckthorn by-products and the antibacterial effects of their extracts on Escherichia coli.The experiment employed the sodium nitrite-aluminum nitrate method to determine flavonoid content,while alcohol extraction and water extraction methods were used to extract active substances from sea buckthorn by-products.The antibacterial effects of sea buckthorn by-product extracts on Escherichia coli were investigated by the filter paper method,perforation method,and broth dilution method.The results showed that the dry matter(DM)content of sea buckthorn by-products was 97.98%,ash content was 3.41%,crude protein(CP)content was 12.87%,and crude fat(EE)content was 22.48%.With the increase of sea buckthorn by-product addition,gas production showed a decreasing trend.Among alcohol extraction and water extraction methods,the constant broth dilution method exhibited the best antibacterial effect.In the alcohol extraction method,the filter paper method showed good antibacterial effect with an inhibition zone of 14.6 mm.In the water extraction method,the filter paper method did not exhibit a significant inhibition zone,and the perforation method was not significant in both alcohol extraction and water extraction methods.The study suggests that sea buckthorn by-products have rich nutritional value,and alcohol extraction is more effective in inhibiting Escherichia coli compared to water extraction.
作者
宁可
黄建智
任智力
刘士哲
王千龙
盛月云
NING Ke;HUANG Jian-zhi;REN Zhi-li;LIU Shi-zhe;WANG Qian-long;SHENG Yue-yun
出处
《饲料研究》
CAS
北大核心
2024年第3期85-89,共5页
Feed Research
基金
国家级大学生创新创业(项目编号:202110757008)
兵团科技创新人才计划(项目编号:2021CB016)(项目编号:2021CB016)。
关键词
沙棘副产品
营养价值
活性提取物
抑菌作用
sea buckthorn by-products
nutritional value
active extract
bacteriostatic action
