摘要
为探究来自中国东北森林凋落物的新发现黄微孢属(Parametarhizium)真菌改善盐碱胁迫下植物生长的作用,利用该属兴安黄微孢菌(P. hingganense)和长白黄微孢菌(P. changbaiense)处理绿豆(Vigna radiata)种子,并对处理后植株分别进行盐、碱胁迫,检测生长表型、光合作用、叶绿素荧光参数、渗透物质含量、活性氧水平、脂质过氧化程度及抗氧化酶活性变化。结果表明:与未处理植株相比,2种真菌处理后绿豆植株在无胁迫和盐、碱胁迫条件下均具有较高生物量。盐、碱胁迫下2种真菌处理植株能够维持较高光合活性,脯氨酸含量增加,丙二醛含量降低,抗氧化酶(POD、SOD、CAT)活性不同程度地升高。综上,黄微孢属的2种真菌通过维护光合系统功能、增加渗透调节物质和增强抗氧化能力来不同程度地改善盐碱胁迫下绿豆植株的生长,其中,兴安黄微孢菌处理能够较显著地减缓盐胁迫损伤,而长白黄微孢菌处理较显著地改善碱胁迫下绿豆生长。
To explore whether the fungi from Parametarhizium,namely P.hingganense or P.changbaiense,a newly discovered fungus from forest litters in northeast China,could improve plant growth under salt and alkali stress,the mung bean seeds were treated with the fungi,followed by the examinations on the phenotypic changes,and alterations on the photosynthesis,chlorophyll fluorescence parameters,osmolyte contents,ROS levels,and antioxidant enzyme activities were detected respectively.The results showed that the biomass of mung bean plants treated with P.hingganense or P.changbaiense were higher under no-stress,salt and alkali stress conditions than untreated plants.Under salt and alkali stress,two fungus-treated plants maintained a high photosynthetic activity,elevated proline content,decreased malondialdehyde(MDA)content,and increased the activities of antioxidant enzymes POD,SOD and CAT in different degrees.In conclusion,through maintenance of photosynthesis,raising osmotic potential,and antioxidant capacity,treatment of the fungi from Parametarhizium alleviated the salt and alkali stress on mung bean.P.hingganense might significantly alleviate salt stress damage,while P.changbaiense might significantly improve the growth of mung bean under alkali stress.
作者
马晨曦
张瑜
孔德馨
高颖
徐利剑
孟威
MA Chenxi;ZHANG Yu;KONG Dexin;GAO Ying;XU Lijian;MENG Wei(Key Laboratory of Saline-alkali Vegetation Ecology Restoration(Northeast Forestry University),Ministry of Education,Harbin 150040;College of Advanced Agriculture and Ecological Environment,Heilongjiang University,Harbin 150080)
出处
《植物研究》
CAS
CSCD
北大核心
2024年第2期239-247,共9页
Bulletin of Botanical Research
基金
黑龙江省自然科学基金资助项目(LH2022C003)
国家自然科学基金项目(31870528)
中央高校基本科研业务费专项资金项目(2572021BD01)。
