摘要
大豆花叶病毒(soybean mosaic virus,SMV)引起的病害是危害大豆生产和品质的主要病害之一,解析大豆抗病遗传机制能为利用大豆抗病品种防治该病害奠定基础。本研究选用193份种质资源,分别接种大豆花叶病毒株系SC7和SC15后进行抗感表型鉴定,结合656977个SNP标记进行全基因组关联定位,结果表明与SC7抗性显著相关位点有一个,位于15号染色体;与SC15抗性显著相关位点有10个,分别位于2、3、4、5、9、12、14、17和19号染色体。这些位点均为新的抗病位点。此外,本研究参考Williams82.a2基因组找出每个抗病位点上下游各50 kb内的基因,利用基因组核苷酸多态性信息,将有SNP落点的基因作为最可能参与抗SMV的候选基因并进行qRT-PCR,分析发现,基因Glyma.02g163300、Glyma.19g027200和Glyma.19g057700可能参与了大豆对SC15的抗性。本研究结果将为大豆抗病种质培育提供重要的基因资源。
Soybean mosaic virus(SMV)disease is one of the main diseases affecting production and quality of soybean.Analyzing the genetic basis of soybean disease resistance can lay a foundation for controlling this disease by using soybean resistant varieties.In this study,193 germplasm resources were performed phenotypic identification by inoculating soybean mosaic virus strains SC7 and SC15,these phenotypic data and 656977 SNP markers of germplasms were used to perform genome-wide association mapping.One significant SNP-trait association with SC7 resistance was detected on chromosome 15.Ten significant SNP-trait association with SC15 resistance were detected and located on chromosomes 2,3,4,5,9,12,14,17 and 19,respectively.These resistance SNP loci maybe new loci.In addition,all genes were found in the upstream and downstream 50 kb of each resistance locus by referring to the Williams82.a2 genome,some genes with SNP difference were selected as the candidate genes for resistance and analyzed by qRT-PCR.The results showed that genes Glyma.02g163300,Glyma.19g027200,and Glyma.19g057700 might be involved in resistance to SC15 in soybean.The results of this study was expected to provide important genetic resources for soybean germplasm cultivation.
作者
牛景萍
杨彩妮
赵晋忠
陈钰涛
王迎新
李丽
王敏
岳爱琴
杜维俊
NIU Jing-ping;YANG Cai-ni;ZHAO Jin-zhong;CHEN Yu-tao;WANG Ying-xin;LI Li;WANG Min;YUE Ai-qin;DU Wei-jun(College of Life Sciences,Shanxi Agricultural University,Jinzhong 030801,China;College of Agronomy,Shanxi Agricultural University,Jinzhong 030801,China;Department of Basic Sciences,Shanxi Agricultural University,Jinzhong 030801,China)
出处
《中国油料作物学报》
CAS
CSCD
北大核心
2024年第1期166-174,共9页
Chinese Journal of Oil Crop Sciences
基金
山西种业创新良种联合攻关项目(2022xczx03)
国家重点研发计划(2021YFD1600601-2)
山西农业大学科技创新基金项目(2018YJ29)
山西省优秀博士来晋工作奖励资金科研项目(SXYBKY2018025)
山西农业大学省部共建有机旱作农业国家重点实验室自主研发项目(202105D121008-3-8)
山西农业大学育种工程项目(YZGC096)
山西农业大学农学院育种工程重点培育专项(YZ2021-05)。
