摘要
本研究旨在以患有隐性乳房炎的奶牛为试验对象,评价蒲公英提取物及其复合酵母培养物对奶牛隐性乳房炎的防治效果。试验选取28头同胎次泌乳牛,其中21头患有隐形乳房炎,以体细胞数量为核心指标,采用Excel随机分为A、B、C三个处理组,保证各组隐性乳房炎患病牛的体细胞数无显著性差异;另7头健康奶牛作为阴性对照D组,每组7个重复,每个重复1头奶牛。试验开始,试验A、B两组分别在基础TMR日粮中添加蒲公英提取物40 g/(d·头)、(40 g蒲公英提取物+150 g酵母培养物)/(d·头),试验C、D两组饲喂基础TMR日粮,试验周期为11 d,分别在第0、6、11天采集牛奶和血液样本,检测牛奶体细胞数、血常规及乳品质等相关指标,同时记录每头牛泌乳量。结果显示:(1)体细胞数,试验第6天,与阳性对照C组相比,日粮添加蒲公英提取物以及蒲公英提取物复配酵母培养物组,牛乳中体细胞数分别下降63.2%和40.1%,二者体细胞数均达到阴性对照D组正常奶牛水平,其中蒲公英提取物组达到显著差异水平(P<0.05),而蒲公英提取物复配酵母培养物组表现出下降趋势(0.05<P<0.1)。试验第11天,牛乳中体细胞数结果显示,与阳性对照C组相比,日粮添加蒲公英提取物以及蒲公英提取物复配酵母培养物组,牛乳中体细胞数分别下降68.0%和79.85%,均达到显著差异水平(P<0.05),且与阴性对照D组相比分别下降52.48%和70.1%,达到显著差异水平(P<0.05)。乳脂率,在试验第0天和第6天,各组乳脂率差异均不显著(P>0.05);试验第11天,与阳性对照C组相比,日粮添加蒲公英提取物A组乳脂率差异不显著(P>0.05),而在日粮中添加蒲公英提取物复合酵母培养物B组乳脂率提升25%,表现出上升的趋势(0.05<P<0.1),且与阴性对照D组相比分别上升18.6%和29.3%,A组表现出上升的趋势(0.05<P<0.1),B组达到显著差异水平(P<0.05)。结果表明,日粮添加蒲公英提取物以及蒲公英提取物复合酵母培养物均可以有效降低牛奶中体细胞数,对治疗隐形乳房炎有显著效果;日粮添加蒲公英提取物复合酵母培养物可以提高牛奶的乳脂率;蒲公英提取物和蒲公英提取物复配酵母培养物对泌乳量、血常规指标无显著影响。
This study aimed to evaluate the preventive and therapeutic effects of dandelion extract compound with yeast culture on subclinical mastitis in dairy cows.Twenty-eight multiparous lactating cows,including 21 diagnosed with subclinical mastitis based on somatic cell count,were randomly assigned to three treatment groups(A,B and C).To ensure comparable somatic cell counts among subclinical mastitis cows in each group,Excel was used for randomization.Additionally,seven healthy cows served as a negative control group(Group D),with seven replicates per group,each consisting of one cow.The experiment,conducted over an 11-day period,involved adding dandelion extract to the basic TMR diet at 40 g/(d·cow)for group A and a combination of 40 g dandelion extract with 150 g yeast culture per day per cow for group B.groups C and D were fed the basic TMR diet.Milk and blood samples were collected on days 0,6 and 11 to analyze somatic cell count,blood routine,and milk quality-related indicators.Simultaneously,milk yield per cow was recorded.The results showed that,on the 6th day,compared to the positive control group C,both dandelion extract and the combination significantly reduced somatic cell counts by 63.2%and 40.1%,respectively.By the 11th day,reductions reached 68.0%and 79.85%,with statistical significance(P<0.05)compared to group C.Milk fat content showed no significant differences among groups on days 0 and 6.On the 11th day,while group A did not show a significant difference,group B demonstrated a 25%increase in milk fat content,indicating a rising trend(0.05<P<0.1).Both A and B groups exhibited an increasing trend compared to the negative control group D(P<0.1),with group B reaching statistical significance(P<0.05).In conclusion,the results indicated that the addition of dandelion extract and its combination with yeast culture effectively reduces somatic cell counts,demonstrating significant therapeutic effects on subclinical mastitis.Moreover,the combination enhances milk fat content.Neither dandelion extract nor its combination with yeast culture significantly affected milk yield or blood routine parameters.
作者
彭清洁
蔡大亮
姜鹏
黄鑫
陈敬帮
周迪
张彦
王苹苹
胡龙飞
戴晋军
PENG Qingjie;CAI Daliang;JIANG Peng;HUANF Xin;CHEN Jingbang;ZHOU Di;ZHANG Yan;WANG Pingping;HU Longfei;DAI Jinjun(National Key Laboratory of Agricultural Microbialogy,Yichang,Hubei Province 443003,China;The Hubei Provincial Key Laboratory of Yeast Function,Yichang,Hubei Province 443003,China;Center for Veterinary Medicine Innovation,China Agricultural University,Beijing 100193,China)
出处
《中国饲料》
2024年第5期166-171,共6页
China Feed
基金
国家重点研发计划资助(2021YFD1301003)。
关键词
隐形乳房炎
酵母培养物
蒲公英提取物
体细胞
单产水平
乳品质
subclinical mastitis
yeast culture
dandelion extract
somatic cell
milk production level
milk quality
