摘要
目的为临床工作中非结核分枝杆菌(NTM)的感染和防治提供科学依据。方法收集疑似结核分枝杆菌感染患者标本(包括呼吸道标本痰液、灌洗液以及非呼吸道标本无菌体液)共1089例,采用DNA微阵列芯片法进行检测,分析分枝杆菌菌种的分布特点。结果1089份标本中检出结核分枝杆菌878份(80.62%),NTM211份(19.38%)。其中非结核分枝杆菌菌种分布有5种,胞内分枝杆菌153例(72.51%),鸟分枝杆菌28例(13.27%),龟脓分枝杆菌复合群19例(9%),堪萨斯分枝杆菌10例(4.74%),耻垢分枝杆菌1例(0.48%)。非结核分枝杆菌感染人员分布男性127例(60.19%),女性84例(39.81%),年龄分布以老年为主,60岁以上103例(48.82%),男性71例(68.93%),女性32例(31.07%)。结论疑似结核分枝杆菌患者检出NTM共有5种,排名前三的NTM种类是胞内分枝杆菌、鸟分枝杆菌和龟脓分枝杆菌复合群,感染人群以老年男性为主。
Objective To investigate the isolation of non-tuberculous mycobacterium(NTM)in Henan Provincial Chest Hospital,and to provide a scientific basis for the infection and prevention of NTM in clinical work in this region.Methods 1089 duplicate specimens(including sputum,lavage solution and sterile bodily fluids of non-respiratory specimens)of suspected mycobacterium tuberculosis infection in Henan Chest Hospital were detected by DNA microarray chip method,and the distribution characteristics of Mycobacterium species were analyzed.Results In 1089 specimens,878(80.62%)of Mycobacterium tuberculosis were detected,and 211(19.38%)were detected in NTM.Among them,there were 5 species of non-tuberculous Mycobacterium species,153 cases(72.51%)were detected in Intracellular Mycobacteria,28 cases(13.27%)of Mycobacterium avium ornithus,19 cases(9%)of Mycobacterium purpureum complex,10 cases(4.74%)of Mycobacterium Kansas,and 1 case(0.48%)of Mycobacterium diureum.Among the 211 specimens,127 males(60.19%),84 female patients(39.81%),and the age distribution was mainly elderly,103 cases over 60 years old(48.82%),including 71 male patients(68.93%)and 32 female patients(31.07%);Conclusions 5 kinds of NTM were detected in patients with suspected Mycobacterium tuberculosis admitted to Henan Chest Hospital,and the top three NTM species were Mycobacterium intracellular,Mycobacterium avium and Mycobacterium turtle complex,and the infected population was mainly elderly men.
作者
谭蛟
王亚春
王亚丽
夏爽
程美锦
王伟
Tan Jiao;Wang Yachun;Wang Yali;Xia Shuang;Cheng Meijin;Wang Wei(Department of Clinical Laboratory,Chest Hospital of Henan Province,Chest Hospital Affiliated to Zhengzhou University,Clinical Research Center for Infectious Diseases(Tuberculosis)of Henan Province,Zhengzhou 450003,China)
出处
《哈尔滨医药》
2024年第1期26-28,共3页
Harbin Medical Journal
关键词
非结核分枝杆菌
菌种鉴定
DNA微阵列芯片技术
Non-mycobacterium tuberculosis
Strain identification
DNA microarray chip technology
