摘要
目的基于氧化应激和炎症探讨健脾解毒汤对银屑病脾虚湿盛证大鼠影响的作用机制。方法将48只6~8周龄,体重180~200 g的SPF级SD雄性大鼠按照随机数字表法分为空白组、模型组、阳性药物对照组及健脾解毒汤低、中、高剂量组,每组8只。空白组背部除毛后涂抹凡士林,其余各组涂抹5%咪喹莫特乳膏(62.5 mg,2次/d)及灌胃大黄颗粒剂(10 ml/kg,2次/d),持续10 d构建银屑病脾虚湿盛证模型。造模成功后,健脾解毒汤低、中、高剂量组分别予2.32、4.64、9.26 g/kg健脾解毒汤灌胃;阳性药物对照组给予甲氨蝶呤片0.97 mg/kg灌胃;空白组、模型组给予等量生理盐水。给药14 d后,比较六组银屑病面积和严重程度指数(PASI)、组织病理情况、白细胞介素-22(IL-22)、丙二醛(MDA)、谷胱甘肽(GSH)含量及超氧化物歧化酶(SOD)活力,比较六组皮损处血红素加氧酶-1(HO-1)蛋白表达情况。结果健脾解毒汤低、中、高剂量组红斑、鳞屑、浸润、进食排便情况优于模型组。健脾解毒汤低、中、高剂量组PASI低于模型组,且健脾解毒汤高剂量组低于健脾解毒汤低、中剂量组(P<0.05)。与模型组比较,健脾解毒汤低、中、高剂量组表皮增生及角化不全减轻,表皮突变短,真皮血管扩张减弱。模型组血清SOD活力、GSH及皮肤组织HO-1水平低于空白组,血清MDA、IL-22水平高于空白组(P<0.05)。健脾解毒汤低、中、高剂量组血清GSH高于模型组,健脾解毒汤中、高剂量组血清SOD活力高于模型组,血清MDA、IL-22低于模型组,健脾解毒汤高剂量组皮肤组织HO-1高于模型组(P<0.05)。健脾解毒汤中、高剂量组血清SOD活力、GSH水平高于健脾解毒汤低剂量组,血清MDA、IL-22水平低于健脾解毒汤低剂量组,健脾解毒汤高剂量组皮肤组织HO-1高于健脾解毒汤低剂量组(P<0.05)。健脾解毒汤高剂量组血清SOD活力、GSH及皮肤组织HO-1水平高于健脾解毒汤中剂量组,血清MDA低于健脾解毒汤中剂量组(P<0.05)。结论健脾解毒汤对银屑病脾虚湿盛证大鼠病变症状具有一定的改善作用,与降低血清中炎症指标,发挥抗感染及抗氧化应激作用相关。
Objective To investigate the mechanism of Jianpi Jiedu Decoction in the treatment of psoriasis rats with syndrome of dampness stagnancy due to spleen deficiency based on oxidative stress and inflammation.Methods A total of 48 SPF grade SD male rats aged 6-8 weeks and weighing 180-200 g were divided into blank group,model group,positive drug control group,and Jianpi Jiedu Decoction low-dose,medium-dose,and high-dose groups according to random number table method,with eight rats in each group.After hair removal,Vaseline was applied to the back of blank group,and other groups were smeared with 5%Imiquimod Cream(62.5 mg,twice a day)and intragastric administration Radixet Rhizoma Rhei Granules(10 ml/kg,twice a day),and the model of psoriasis with syndrome of dampness stagnancy due to spleen deficiency was established for ten days.After successful modeling,the Jianpi Jiedu Decoction low-dose,medium-dose,and high-dose groups were given 2.32,4.64,and 9.26 g/kg Jianpi Jiedu Decoction,respectively.The positive control group was given 0.97 mg/kg Methotrexate Tablets by intragastric administration;blank group and model group were given the same amount of normal saline.After 14 days of administration,psoriasis area and severity index(PASI),histopathology,contents of interleukin-22(IL-22),malondialdehyde(MDA),glutathione(GSH),activity of superoxide dismutase(SOD),and expression of heme oxygenase-1(HO-1)protein were compared among the six groups.Results The erythema,scale,infiltration,feeding,and defecation of Jianpi Jiedu Decoction low-dose,medium-dose,and high-dose groups were better than those in model group.PASI in Jianpi Jiedu Decoction low-dose,medium-dose,and high-dose groups were lower than those in model group,PASI in Jianpi Jiedu high-dose group was lower than that in Jianpi Jiedu Decoction low-dose,medium-dose groups(P<0.05).Compared with the model group,the epidermal hyperplasia and hyperkeratosis of Jianpi Jiedu Decoction low-dose,medium-dose,and high-dose groups were reduced,epidermal mutation was shorter,and dermal vasodilation was weakened.SOD activity,GSH and HO-1 levels in model group were lower than those in blank group,MDA and IL-22 levels were higher than those in blank group(P<0.05).The serum levels of GSH in Jianpi Jiedu Decoction low-dose,medium-dose,and high-dose groups were higher than those in model group,serum SOD activity in Jianpi Jiedu Decoction medium-dose,high-dose groups were higher than those in model group,serum MDA and IL-22 were lower than those in model group,and skin tissue HO-1 in Jianpi Jiedu Decoction high-dose group was higher than that in model group(P<0.05).Serum SOD activity and GSH level in Jianpi Jiedu Decoction medium-dose and high-dose groups were higher than those in Jianpi Jiedu Decoction low-dose group,serum MDA and IL-22 levels were lower than those in Jianpi Jiedu Decoction low-dose group,and skin tissue HO-1 in Jianpi Jiedu Decoction high-dose group was higher than that in Jianpi Jiedu Decoction low-dose group(P<0.05).The serum SOD activity,GSH,and skin tissue HO-1 levels in Jianpi Jiedu Decoction high-dose group were higher than those in Jianpi Jiedu Decoction medium-dose group,and serum MDA was lower than those in Jianpi Jiedu Decoction medium-dose group(P<0.05).Conclusion Jianpi Jiedu Decoction can improve the pathological symptoms of psoriasis rats with syndrome of dampness stagnancy due to spleen deficiency,and it is related to reducing the inflammatory index in serum,playing the role of anti-infection and anti-oxidative stress.
作者
许孟月
姚文汇
左永杰
刘红霞
XU Mengyue;YAO Wenhui;ZUO Yongjie;LIU Hongxia(The Fourth Clinical Medical College,Xinjiang Medical University,Xinjiang Uygur Autonomous Region,Urumqi 830000,China;Department of Dermatology,the Affiliated Hospital of Traditional Chinese Medicine,Xinjiang Uygur Autonomous Region,Urumqi 830000,China)
出处
《中国医药导报》
CAS
2024年第5期6-10,23,共6页
China Medical Herald
基金
国家自然科学基金地区科学基金资助项目(81960871)
新疆维吾尔自治区重点研发计划项目(2021B03001-2)。
关键词
银屑病
健脾解毒汤
氧化应激
炎症
Psoriasis
Jianpi Jiedu Decoction
Oxidative Stress
Inflammation
