摘要
小麦赤霉病是影响小麦产量与粮食安全的重大真菌病害之一,其主要致病菌为禾谷镰孢(Fusarium graminearum)。染色质缩合调节因子(RCC1)作为一种核蛋白,能促进Ran结合的GDP/GTP转换,在染色质缩合起始、核质转运和有丝分裂过程中起关键作用,但其在丝状真菌中的功能尚未见报道。本研究通过基因敲除的方法,获得了禾谷镰孢中基因FgRCC1的敲除突变体ΔFgRCC1和互补菌株ΔFgRCC1-C。通过表型测定分析,发现敲除突变体ΔFgRCC1的生长速率较野生型菌株PH-1和ΔFgRCC1-C降低约8.5%,且菌丝分枝变多。敲除突变体ΔFgRCC1的分生孢子产量降低约8.8%,并且隔膜数在4和5个的分生孢子比例明显增多。此外,敲除突变体ΔFgRCC1对杀菌剂戊唑醇变得敏感,而对多菌灵、刚果红、SDS、KCl、CaCl_(2)、MgCl_(2)的抗性增强。致病性测定发现,ΔFgRCC1在小麦上的致病力下降,并且其体内毒素体形成受阻,DON毒素生物合成显著减少,7种TRIs转录水平显著降低。研究结果表明,染色质缩合调节因子FgRCC1在禾谷镰孢菌丝生长、参与逆境胁迫以及致病和产毒过程中有重要作用。
Fusarium head blight is one of the major fungal diseases affecting the yield of wheat and food security.The main pathogen is Fusarium graminearum.Regulator of chromatin condensation(RCC1)is a nuclear protein that promotes the Ran-bound GDP/GTP exchange and plays a significant role in chromosome condensation onset,nucleo-cytoplasmic transport and mitosis.However,function of RCC1 in filamentous fungi remains unclear until now.In this study,FgRCC1 gene null mutantΔFgRCC1 and complementary strainΔFgRCC1-C in F.graminearum,was obtained by gene disruption approach.Phenotype analysis showed that the growth rate ofΔFgRCC1 was about 8.5%slower than that of wild type PH-1 andΔFgRCC1-C,but had more branching in the hypha.Conidiation ofΔFgRCC1 mutant decreased by 8.8%and percentage of conidia with 4 to 5 septa was significantly increased.In addition,ΔFgRCC1 became sensitive to tebuconazole,but more resistant to carbendazim,congo red,SDS,KCl,CaCl_(2),and MgCl_(2).Pathogenicity assays indicated that the virulence ofΔFgRCC1 on wheat was seriously reduced,and the formation of toxisome was blocked resulting in dramatic reduction of DON biosynthesis,the transcription levels of seven TRIs were significantly reduced.Taken together,these results indicate that FgRCC1 plays important roles in the regulation of fungal growth,virulence and DON biosynthesis,as well as various stress responses in F.graminearum.
作者
王亚轩
张承启
陈莉
WANG Yaxuan;ZHANG Chengqi;CHEN Li(Anhui Province Key Laboratory of Integrated Pest Management on Crops,College of Plant Protection,Anhui Agricultural University,Hefei 230036,China)
出处
《植物病理学报》
CAS
CSCD
北大核心
2023年第6期1096-1106,共11页
Acta Phytopathologica Sinica
基金
国家重点研发计划“小麦赤霉病灾变机制与可持续防控技术研究”(2022YFD1400100)。
关键词
禾谷镰孢
染色质缩合调节因子
生长发育
逆境胁迫
致病性
Fusarium graminearum
regulator of chromatin condensation
growth and development
environmental stress
pathogenicity
