摘要
目的 探讨微RNA-296(mi R-296)、血管内皮生长因子-B(VEGF-B)的表达与冠心病(CHD)患者冠脉病变程度的关系。方法 选取2019年1月至2022年11月山东省聊城市第二人民医院收治的93例CHD患者,根据冠状动脉病变支数分为单支病变组38例、双支病变组33例、多支病变组22例,再根据Gensini积分将其分为冠状动脉狭窄(CAS)轻度组30例、中度组34例、重度组29例。采用实时荧光定量聚合酶链式反应检测mi R-296,酶联免疫吸附试验检测血清VEGF-B。通过双萤光素酶报告基因验证mi R-296与VEGF-B的关系。采用Pearson相关性分析CHD患者mi R-296、VEGF-B表达的相关性,采用Spearman相关性分析CHD患者mi R-296、VEGF-B表达与Gensini积分及冠脉病变支数的相关性。结果 多支病变组mi R-296表达低于单支病变组和双支病变组,VEGF-B水平高于单支病变组和双支病变组,且双支病变组mi R-296表达低于单支病变组,VEGF-B高于单支病变组,差异有统计学意义(P<0.05)。重度组mi R-296表达低于轻度组和中度组,VEGF-B水平高于轻度组和中度组,且中度组mi R-296表达低于轻度组,VEGF-B水平高于轻度组,差异有统计学意义(P<0.05)。双萤光素酶报告基因显示,VEGF-B是mi R-296的靶点。Pearson相关性分析显示,CHD患者mi R-296与VEGF-B表达呈负相关(r=-0.731,P<0.05)。Spearman相关性分析显示,CHD患者mi R-296表达与Gensini积分及病变支数呈负相关(rs=-0.719、0.638,P<0.05),VEGF-B表达与Gensini积分及病变支数呈正相关(rs=0.727、0.698,P<0.05)。结论CHD患者mi R-296低表达,VEGF-B高表达,两者与CAS病变程度密切相关。
Objective To investigate relationship between the expression of micro RNA-296(miR-296),vascular endothelial growth factor-B(VEGF-B) and the degree of coronary artery disease in patients with coronary heart disease(CHD).Methods A total of 93 patients with CHD admitted to the Second People's Hospital of Liaocheng from January 2019to November 2022 were selected.They were divided into single vessel lesion group of 38 cases,dual vessel lesion group of33 cases,and multi vessel lesion group of 22 cases based on the number of coronary artery lesions.According to the Gensini score,they were divided into coronary artery stenosis(CAS) mild group of 30 cases,moderate group of 34 cases,and severe group of 29 cases.Real-time fluorescence quantitative polymerase chain reaction was used to detect miR-296,and enzyme linked immunosorbent assay was used to detect serum VEGF-B.The relationship between miR-296 and VEGF-B was verified using dual luciferase reporter genes.Pearson correlation was used to analyze the correlation between miR-296 and VEGF-B expression in CHD patients,and Spearman correlation was used to analyze the correlation between miR-296 and VEGF-B expression,Gensini score,and the number of coronary lesions in CHD patients.Results The expression of miR-296 in the multi vessel lesion group was lower than that in the single vessel lesion group and dual vessel lesion group,and the level of VEGF-B was higher than that in the single vessel lesion group and dual vessel lesion group,the expression of miR-296 in the dual vessel lesion group was lower than that in the single vessel lesion group,and VEGF-B was higher than that in the single vessel lesion group,and the differences were statistically significant(P<0.05).The expression of miR-296 in the severe group was lower than that in the mild and moderate groups,and the level of VEGF-B was higher than that in the mild and moderate groups,the expression of miR-296 in the moderate group was lower than that in the mild group,and the level of VEGF-B was higher than that in the mild group,and the differences were statistically significant(P<0.05).The dual luciferase reporter genes showed that VEGF-B was the target of miR-296.Pearson correlation analysis showed a negative correlation between miR-296 and VEGF-B expression in CHD patients(r=-0.731,P<0.05),Spearman correlation analysis showed a negative correlation between miR-296 expression and Gensini score and lesion number in CHD patients(rs=-0.719,0.638,P<0.05),and a positive correlation between VEGF-B expression and Gensini score and lesion number(rs=0.727,0.698,P<0.05).Conclusion The low expression of blood miR-296 and high expression of VEGF-B in CHD patients are closely related to the degree of CAS lesions.
作者
李震
郝恩刚
刘丹青
LI Zhen;HAO Engang;LIU Danqing(Department of Clinical Laboratory,the Second People’s Hospital of Liaocheng,Shandong Province,Linqing252600,China;Department of Cardiology,the Second People’s Hospital of Liaocheng,Shandong Province,Linqing252600,China;Department of Clinical Laboratory,Linqing People’s Hospital,Shandong Province,Linqing252600,China)
出处
《中国医药导报》
CAS
2024年第4期11-15,共5页
China Medical Herald
基金
山东省医药卫生科技发展计划项目(202103010807)。
