摘要
目的了解吉林省食品中沙门氏菌的分布情况和耐药基因特征,为防控食源性疾病提供科学依据。方法对2022年吉林省食品中61株沙门菌,采用微量肉汤稀释法进行25种抗生素耐药性试验。利用全基因组测序技术及生物信息学方法对菌株耐药基因进行分析。结果61株沙门菌分为19种血清型,优势血清型为肠炎沙门菌(52.46%,32/61)。耐药性分析结果显示,沙门菌对氨苄西林的耐药率最高(60.66%,37/61),多重耐药率达24.60%(15/61),无优势耐药谱。不同抗生素的耐药表型与耐药基因存在差异。结论吉林省食品中沙门氏菌的多重耐药比例较高,耐药谱模式复杂,耐药基因携带率较高,需加强耐药性监测,避免抗生素滥用。
This study was aimed at understanding the distribution and drug-resistance gene characteristics of Salmonella from food products in Jilin Province,to provide a scientific basis for the prevention and control of food-borne diseases.A total of 61 strains of Salmonella isolated from food products collected from Jilin Province in 2022 were tested for resistance to 25 antibiotics with the micro-broth dilution method.Resistance genes in the strains were analyzed with whole genome sequencing and bio-informatics methods.The 61 strains of Salmonella were divided into 19 serotypes,among which the dominant serotype was Enteritidis(52.46%,32/61).The results of resistance analysis indicated that the highest resistance rate was 60.66%,(37/61),for ampicillin,and the multi-drug resistance rate was 24.60%(15/61).No dominant drug resistance spectrum was observed.Differences in resistance phenotype and resistance genes were found among antibiotics.Salmonella from food in Jilin Province had a high proportion of multi-drug resistance,showed complex drug resistance spectrum patterns,and had a high carriage rate of drug resistance genes.Thus,efforts in drug resistance monitoring and avoiding antibiotic misuse must be strengthened.
作者
孙景昱
赵薇
孙绩岩
石奔
李可维
黄鑫
SUN Jing-yu;ZHAO Wei;SUN Ji-yan;SHI Ben;LI Ke-wei;HUANG Xin(Jilin Provincial Center for Disease Control and Prevention(Jilin Public Health Research Institute),Changchun 130062,China;Nongan County People’s Hospital,Changchun 130200,China)
出处
《中国人兽共患病学报》
CAS
CSCD
北大核心
2024年第2期104-110,共7页
Chinese Journal of Zoonoses
基金
吉林省卫生与健康科技创新项目(No.2020Z016)
吉林省科技发展计划项目(No.20210204139YY)联合资助。
关键词
沙门氏菌
食品
血清分型
耐药基因
全基因组测序
Salmonella
food
serotyping
resistance genes
whole-genome sequencing(WGS)
