摘要
目的 探讨中性粒细胞CD11b (nCD11b)、单核细胞CD14 (mCD14)和单核细胞CD86 (mCD86)表达对新生儿败血症病情严重程度的预测价值。方法 选取2018年2月至2022年2月南阳市中心医院收治的73例新生儿败血症作为疾病组,根据患儿是否发生休克分为休克组26例和非休克组47例,另选取同期体检的足月健康新生儿73例为健康组。采用流式细胞法检测所有新生儿的外周血n CD11b、m CD14和m CD86表达水平,比较各组新生儿外周血n CD11b、mCD14和m CD86表达水平,并采用受试者工作特征曲线(ROC)分析其对新生儿败血症病情程度的预测价值。结果 疾病组新生儿的外周血n CD11b、mCD86表达水平分别为(220.00±12.58) MFI、(62.89±7.69) MFI,明显高于健康组的(186.69±10.98) MFI、(41.27±5.09) MFI,而外周血m CD14表达水平为(38.85±6.27) MFI,明显低于健康组的(54.03±6.15) MFI,差异均有统计学意义(P<0.05);休克组新生儿的外周血nCD11b、m CD86表达水平分别为(227.69±11.62) MFI、(67.96±6.18) MFI,明显高于非休克组的(215.74±12.95) MFI、(60.08±8.29) MFI,而外周血m CD14表达水平为(34.99±5.83) MFI,明显低于非休克组的(40.98±6.54) MFI,差异均有统计学意义(P<0.05);经ROC分析结果显示,外周血nCD11b、m CD14、mCD86单独及其联合检测对新生儿败血症病情程度的曲线下面积(AUC)分为0.850、0.804、0.815和0.930,联合检测AUC均高于其单独检测(P<0.05)。结论 外周血n CD11b、m CD14、m CD86检测可用于新生儿败血症病情严重程度评估,且联合检测可提高新生儿败血症病情严重程度预测价值。
Objective To explore the predictive value of neutrophil CD11b(nCD11b),monocyte CD14(mCD14),and monocyte CD86(m CD86) for the severity of neonatal sepsis.Methods Seventy-three cases of neonatal sepsis admitted to Nanyang Central Hospital from February 2018 to February 2022 were selected as the disease group.Children in the disease group were divided into a shock group of 26 cases and a non-shock group of 47 cases based on whether they experienced shock.In addition,73 full-term healthy newborns who underwent physical examinations during the same period were selected as the healthy group.Flow cytometry was used to detect the expression levels of n CD11b,m CD14,and m CD86 in the peripheral blood of all newborns.The expression levels of n CD11b,m CD14,and m CD86 in the peripheral blood of each group of newborns were compared,and the receiver operating characteristic curve(ROC) was used to analyze their predictive value for the severity of neonatal sepsis.Results The expression levels of n CD11b and mCD86 in the peripheral blood of newborns in the disease group were(220.00± 12.58) MFI and(62.89±7.69) MFI,respectively,significantly higher than(186.69±10.98) MFI and(41.27±5.09) MFI in the healthy group,while the expression level of m CD14 in the peripheral blood was(38.85±6.27) MFI,significantly lower than(54.03±6.15) MFI in the healthy group,with statistically significant differences(P<0.05).The expression levels of nCD11b and mCD86 in the peripheral blood of newborns in the shock group were(227.69±11.62) MFI and(67.96±6.18) MFI,respectively,significantly higher than(215.74±12.95) MFI and(60.08±8.29) MFI in the non-shock group;the expression level of m CD14 in peripheral blood was(34.99±5.83) MFI,which was significantly lower than(40.98±6.54) MFI of the non-shock group;the differences were statistically significant(P<0.05).ROC analysis results showed that the area under the curve(AUC) of peripheral blood nCD11b,mCD14,m CD86,and their combined detection for the severity of neonatal sepsis were 0.850,0.804,0.815,and 0.930,respectively.The AUC of combined detection was significantly higher than that of single detection(P<0.05).Conclusion The peripheral blood n CD11b,m CD14,and m CD86can evaluate the severity of neonatal sepsis,and the combined detection can improve the predictive value.
作者
张义堂
秦小菀
石岩
侯海燕
殷雪
徐会民
ZHANG YI-tang;QIN Xiao-wan;SHI Yan;HOU Hai-yan;YIN Xue;XU Hui-min(Department of Pediatrics,Nanyang Central Hospital,Nanyang 473000,Henan,CHINA)
出处
《海南医学》
CAS
2024年第5期709-712,共4页
Hainan Medical Journal
基金
2019年度河南省医学科技攻关计划联合共建项目(编号:LHGJ20190913)。
