摘要
目的 探讨高级别浆液性卵巢癌(HGSOC)的差异表达基因(DEGs)及其临床意义。方法 从GEO数据库下载与HGSOC相关的基因芯片数据集GSE54388,使用R语言筛选DEGs,并采用DAVID数据库进行富集分析。选取35例HGSOC患者的组织标本(包含卵巢癌组织及距离癌组织>1 cm处的癌旁组织),采用实时荧光定量PCR检测DEGs在卵巢癌组织和癌旁组织中的表达水平。利用STRING数据库和Cytoscape 3.8.0软件构建蛋白-蛋白相互作用(PPI)网络并筛选核心基因。结果 在GSE54388数据集中共筛选出537个DEGs,其中表达下调基因369个,表达上调基因168个。实时荧光定量PCR结果显示,卵巢癌组织PEX5L、ITLN1和SLC4A4的mRNA表达水平低于癌旁组织(P<0.05)。富集分析结果显示,DEGs富集于细胞外液、细胞外基质等细胞组分上,涉及肝素结合、钙离子结合等分子功能,参与细胞黏附、分裂和增殖等生物过程,与Ras信号通路、癌症相关通路等密切相关。从PPI网络中筛选出10个核心基因,即CDK1、CDC20、cyclin B1、BUB1B、KIF20A、DLGAP5、NDC80、NCAPG、TTK和KIF11。结论 PEX5L、ITLN1和SLC4A4可能是影响HGSOC发生发展的关键DEGs, Ras信号通路、磷脂酰肌醇3-激酶信号通路、经典Wnt信号通路可能参与调控HGSOC的进展。
Objective To explore the differentially expressed genes(DEGs)in high-grade serous ovarian cancer(HGSOC)and its clinical significance.Methods A data set of gene chips GSE54388 related to HGSOC was downloaded from the GEO database,DEGs were screened by using the R language software,and enrichment analyses were performed by using the DAVID database.A total of 35 tissue samples of HGSOC patients were selected,including ovarian cancerous tissues and paracancerous tissues>1 cm away from cancerous tissues,and the real-time fluorescent quantitative PCR was employed to detect DEGs expressions in ovarian cancerous tissues and paracancerous tissues.The STRING database and Cytoscape 3.8.0 software were used to establish protein-protein interaction(PPI)network for screening the core genes.Results A total of 537 DEGs were screened in GSE54388 data set,therein,there were 369 down-regulated expressed genes and 168 up-regulated expressed genes.The results of real-time fluorescent quantitative PCR revealed that mRNA expressions of PEX5L,ITLN1,and SLC4A4 in ovarian cancerous tissues were lower than those in paracancerous tissues(P<0.05).The results of enrichment analyses depicted that DEGs were enriched in cellular compositions of extracellular space and extracellular matrix,etc.,were involved in molecular functions of heparin binding and calcium ion binding,etc.,participated in biological processes in terms of cellular adhesion,division,and proliferation,etc.,and were related to Ras signaling pathway,pathway in cancer,etc.A total of 10 core genes were screened from PPI network,namely,CDK1,CDC20,cyclin B1,BUB1B,KIF20A,DLGAP5,NDC80,NCAPG,TTK,and KIF11.Conclusion PEX5L,ITLN1,and SLC4A4 may be the key DEGs for affecting the occurrence and development of HGSOC,and Ras signaling pathway,phosphoinositide 3-kinase signaling pathway,canonical Wnt signaling pathway may be involved in regulating HGSOC progression.
作者
高美
尉春艳
闫桂花
刘亮亮
GAO Mei;WEI Chunyan;YAN Guihua;LIU Liangliang(Department of Gynecology and Obstetrics,the Second Affiliated Hospital of Xi′an Jiaotong University,Xi′an 710000,Shaanxi,China;Department of Emergency,the Second Affiliated Hospital of Xi′an Jiaotong University,Xi′an 710000,Shaanxi,China)
出处
《广西医学》
CAS
2023年第24期3006-3011,共6页
Guangxi Medical Journal
基金
陕西省自然科学基础研究计划面上项目(2019JZ-46)。
关键词
高级别浆液性卵巢癌
差异表达基因
生物信息学分析
High-grade serous ovarian cancer
Differentially expressed genes
Bioinformatics analysis
