摘要
目的 探讨电针(EA)在急性痛风性关节炎大鼠模型通过调控瞬时受体电位香草酸亚型1(TRPV1)对疼痛反应的影响。方法 60只SPF级雄性SD大鼠分为4组,即对照组、模型组、假EA治疗组和EA治疗组,每组15只。通过向大鼠的脚踝注射尿酸钠以建立痛风模型。EA治疗组分别在模型建立后第8、24和48 h进行EA治疗。假EA治疗组将针头皮下插入穴位,但没有放电。对照组和模型组不接受治疗。分别对各组大鼠的持续疼痛评分、机械性疼痛、热痛觉过敏进行测定,采用western blot分析大鼠外周背根神经节与中枢脊髓背角中TRPV1和相关下游分子的表达。结果 与假EA治疗组相比,在8、24和48 h时间点,EA治疗组显著降低了痛风大鼠的持续疼痛评分(P<0.05),同时显著增加了50%爪退缩阈值(PWT)和爪退缩潜伏期(P<0.05)。无论在背根神经节与中枢脊髓背角,与对照组相比,模型组TRPV1和相关的下游蛋白激酶(S100B、GFAP和RAGE)水平表达均显著提高(P<0.05)。EA治疗显著降低了TRPV1和相关的下游蛋白激酶(S100B、GFAP和RAGE)的过表达(P<0.05),而假EA治疗并没有改变这一观察结果。结论 EA在急性痛风性关节炎大鼠模型中可有效缓解疼痛反应,其主要通过TRPV1及其下游蛋白激酶S100B、GFAP和RAGE受体介导镇痛作用。
Objective To investigate the effects of electroacupuncture(EA) in a rat model with acute gout arthritis on pain response via the regulation transient receptor potential vanillic acid subtype 1(TRPV1).Methods 60 rats were divided into 4 groups(n=15):control group,model group,sham EA treatment group and EA treatment group.Acute gout arthritis model was established by injecting sodium urate into the ankle of rats.EA treatment group was treated with EA at the 8h,24h and 48h after the establishment of the model.For Sham EA treatment,the rats were inserted with needles subcutaneously into acupoints,but with no electrical discharge.The control group and the model group were not treated.The persistent pain score,mechanical pain and thermal hyperalgesia were measured,and the expression of TRPV1 and related downstream molecules in peripheral dorsal root ganglion and central spinal dorsal horn were analyzed by Western blot.Results Compared with sham EA group,EA treatment at 8 h,24 h and 48 h significantly reduced the persistent pain score of gout rats(P<0.05),and significantly increased the latency of 50% PWT and claw retraction(P<0.05).Compared with the control group,the expression of TRPV1 and related downstream protein kinase(S100B,GFAP and RAGE) in the model group was significantly increased(P<0.05).EA treatment significantly reduced the overexpression of TRPV1 and related downstream protein kinases(S100B,GFAP and RAGE)(P<0.05),while Sham EA treatment did not alter this observation.Conclusion EA treatment can alleviate pain response partly via the regulation TRPV1 and its downstream protein kinase S100B,GFAP and RAGE receptor in a rat model of acute gout arthritis.
作者
李赟
张如峰
吴文英
LI Yun;ZHANG Rufeng;WU Wenying(Department of Rheumatology,Xining First People's Hospital,Xining 810000,China)
出处
《西部医学》
2024年第3期351-356,共6页
Medical Journal of West China
基金
青海省自然科学基金研究计划项目(20190135)。
关键词
电针
香草酸亚型1
急性痛风性关节炎
疼痛反应
Electroacupuncture
Vanillic acid subtype 1
Acute gouty arthritis
Pain response
