摘要
目的:研究P16^(INK4a)在宫颈炎症与宫颈鳞状上皮内病变以及癌组织中的免疫表达及其与临床病理特征和HPV-DNA分型的相关性。方法:收集商丘市第一人民医院2020年4月至2023年4月404例宫颈病变组织病理活检,包括炎症组织、宫颈鳞状上皮内病变以及鳞状细胞癌(SCC);统计所有病例的P16^(INK4a)免疫组化染色与HPV-DNA分型结果,并分析各病变组织中的P16^(INK4a)与HPV分型关系。结果:P16^(INK4a)的表达在各种宫颈病变组织中存在显著差异(P<0.05),且P16^(INK4a)的阳性率与表达强度随宫颈病变程度的加深而增加;宫颈组织中P16^(INK4a)的表达在低危型与高危型HPV间有显著差异(P<0.05),P16^(INK4a)的表达强度与高危型HPV有相关性(P<0.001)。结论:P16^(INK4a)免疫染色结合HPV-DNA分型能有助于鉴别高级别CIN和宫颈癌,以及区分这些病变与低级别CIN和正常宫颈组织。
Objective:To study the immunoexpression of P16^(INK4a) in cervical inflammation,squamous intraepithelial lesions and cancer tissues and its correlation with clinicopathological features and HPV-DNA typing.Methods:Pathological biopsies of 404 cases from the first people's of Shangqiu City from April 2020 to April 2023 were collected,including inflammatory tissue,cervical squamous intraepithelial lesions and squamous cell carcinoma(SCC).The results of P16^(INK4a) immunohistochemical staining and HPV-DNA typing in all cases were analyzed,and the relationship between P16^(INK4a) and HPV typing in each pathological tissue was analyzed.Results:There were significant differences in the expression of P16^(INK4a) in various cervical lesions(P<0.05),and the positive rate and expression intensity of P16^(INK4a) increased with the deepening of cervical lesions(P<0.05).The expression of P16^(INK4a) in cervical tissues was significantly different between low-risk and high-risk HPV infection,and the expression intensity of P16^(INK4a) was correlated with high-risk HPV(P<0.001).Conclusion:P16^(INK4a) immunostaining combined with HPV-DNA typing can help distinguishing high-grade CIN from cervical cancer,and distinguish these lesions from low-grade CIN and normal cervical tissue.
作者
牛爱琴
杨苗苗
朱冰
王艳春
竞柏松
NIU Aiqin;YANG Miaomiao;ZHU Bing;WANG Yanchun;JING Baisong(Department of Gynecology,the First People's Hospital of Shangqiu,Shangqiu 476100,China)
出处
《黑龙江医药科学》
2024年第1期71-74,共4页
Heilongjiang Medicine and Pharmacy
基金
河南省医学科技攻关计划(联合共建)项目,编号:LHGJ20191494。
